Simultaneous determination of schisantherin and deoxyschizandrin in Wuweizi syrup by HPLC
SONG Bo1, SUN Yukun1, TIAN Jie2, SONG Yang3, TANG Zhe1, YAO Hui1, WANG Haipeng1
1.Liaoning Provincial Crops Hospital,Chinese People’s Armed Police Force,Shenyang 110034,China. 2. Chinese Medicine Hospital of Tangshan,Tangshan 063000, China. 3.Medical Team, Troop 93321 of PLA,Shenyang 10031, China
Abstract：Objective To establish a method for the determination of schisantherin and deoxyschizandrin in Wuweizi syrup.Methods The high performance liquid chromatography method was used and the separation was performed on an Eclipse Plus C18(3.5 μm,4.6×100 mm)with acetonitrile-0.1% phosphoric acid solution(60∶40) as the mobile phase with gradient elution at a flow rate of 1.2 ml/min. The detection wavelength was set at 254 nm, and the column temperature was 26 ℃.Results The linear range of schisantherin and deoxyschizandrin was 1.04-26.23 μg (r=0.9991) and 1.05-26.51 μg (r=0.9995)respectively. The average recovery was 99.58％ (RSD=1.72％,n=6) and 99.95％ (RSD=1.29％,n=6), respectively.Conclusions The established HPLC method is proved to be simple, reproducible, precise and suitable for the content determination of Wuweizi syrup.
宋博, 孙玉坤, 田洁, 宋旸, 唐哲, 姚辉, 王海鹏. HPLC法同时测定五味子糖浆中五味子酯甲和五味子甲素的含量[J]. 武警医学, 2018, 29(3): 267-269.
SONG Bo, SUN Yukun, TIAN Jie, SONG Yang, TANG Zhe, YAO Hui, WANG Haipeng. Simultaneous determination of schisantherin and deoxyschizandrin in Wuweizi syrup by HPLC. Med. J. Chin. Peop. Armed Poli. Forc., 2018, 29(3): 267-269.
2018, Vol. 29 
