Applicability of ICG nanoprobes in fluorescence molecular imaging of colon cancer
LIU Zhichao1,QU Yawei2, LIU Haifeng3.
1.Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China;
2.Department of Gastroenterology;
3.Department of Medical Administration,General Hospital of Chinese People’s Armed Police Force, Beijing 100039,China
Objective To evaluate the targeting and fluorescence effect of ICG nanoprobes in fluorescent molecular imaging of colon cancer, and to explore a new method for early diagnosis of colon cancer.Methods A nude mouse model of human colon cancer xenograft was established. An ICG nano probe packaged by human serum albumin (HSA) was used, with Folate RsenseTM680 and indocyanine green as the control group. After intravenous injection of the probe into the colon cancer xenograft model in nude mice, the in vivo fluorescence molecular imaging (FLI) was used to observe the HAS-ICG effect of FLI nanoparticles. The fluorescence signal intensity of tumor was quantitatively analyzed.Results In vivo fluorescence molecular imaging showed that the concentration of HAS-ICG and Folate RsenseTM680 in the subcutaneous tumor sites of experimental mice reached the peak 1 h and 24 h after injection respectively. Compared with the ICG control group, the HAS-ICG nanoprobe had a better target labeling ability and signal-to-noise ratio than the commercial probe.Conclusions The subcutaneous model of nude mouse colon cancer injected with an HSA-ICG nanoparticle probe has obvious FLI imaging effect. It has good ability to mark HCT116 colonic tumor cells, and can be used to diagnose early colon cancer in nude mice by means of fluorescence molecular imaging.
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2018, Vol. 29 
