Biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells
DENG Banglian1,2, CHEN Xuefeng1, MENG Lei1, YI Zian1, QIN Wen3, LIU Yan3, SONG Shuang4, PENG Chao1, and JU Yanjing1.
1.Department of Orthodontics, Lianbang Dental Hospital of Xincheng District, Xi’an 710000,China; 2.Department of Stomatology, People’s Hospital of Ankang, Ankang 725000,China; 3.Department of Orthodontics, School of Stomatology, the Fourth Military Medical University, Xi’an 710032 China; 4.Peking University School of Stomatology, Beijing 100191,China
Abstract：Objective To evaluate the biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells.Methods The hydrodynamic size and zeta potencial of GQDs in distilled water (DW) were determined by dynamic light scattering (DLS). Furthermore, cell survival rates following treatment with GQDs were evaluated using the CCK-8 assay and the biological imaging effect of GQDs in PC12 cells was observed under a confocal microscope.Results The toxic effect of GQDs in PC12 cells was size-dependent. The results of CCK-8 assay indicated that 15 nm GQDs showed lower toxicity than CDs in PC12 cells. After PC12 cells were incubated 48 h with 500 μg/ml GQDs, cell viability remained at 80% or more. What’s more, 15 nm GQDs could be taken up by PC12 cells more easily and more than 80% cells could be labeled successfully, which indicated good biological imaging effects.Conclusions GQDs have low cytotoxicity and excellent biological imaging properties, which are a suitable nanomaterial for neuroimaging.
邓邦莲, 陈雪峰, 孟蕾, 易子安, 秦文, 刘龑, 宋爽, 彭超, 巨妍静. 不同粒径的石墨烯量子点在PC12细胞中的成像效应及细胞毒性[J]. 武警医学, 2018, 29(9): 901-904.
DENG Banglian, CHEN Xuefeng, MENG Lei, YI Zian, QIN Wen, LIU Yan, SONG Shuang, PENG Chao, and JU Yanjing.. Biological imaging effect and biocompatibility of graphene quantum dots (GQDs) of different size in PC12 cells. Med. J. Chin. Peop. Armed Poli. Forc., 2018, 29(9): 901-904.
Michalet X, Pinaud F F, Bentolila L A, et al. Quantum dots for live cells, in vivo imaging, and diagnostics[J]. Science, 2005, 307(5709): 538-544.
[2]
Xu Y, Wang X, Zhang W L, et al. Recent progress in two-dimensional inorganic quantum dots[J]. Chem Soc Rev, 2018, 47(2): 586-625.
[3]
Li L, Wu G, Yang G, et al. Focusing on luminescent graphene quantum dots: current status and future perspectives[J]. Nanoscale, 2013, 5(10): 4015-4039.
[4]
Yoo J M, Kang J H, Hong B H. Graphene-based nanomaterials for versatile imaging studies[J]. Chem Soc Rev, 2015, 44(14): 4835-4852.
[5]
Tan D, Zhou S, Qiu J. Comment on “Upconversion and Downconversion Fluorescent Graphene Quantum Dots: Ultrasonic Preparation and Photocatalysis” [J]. Acs Nano, 2012, 6(8):1059-1064.
[6]
Xu G, Zeng S, Zhang B, et al. New generation cadmium-free quantum dots for biophotonics and nanomedicine[J]. Chem Rev, 2016, 116(19):12234.
[7]
Halamodakenzaoui B, Ceridono M, Colpo P, et al. Dispersion behaviour of silica nanoparticles in biological media and its influence on cellular pptake[J]. PLoS One, 2015,10(10):0141593.
[8]
Markovic Z M, Ristic B Z, Arsikin K M, et al. Graphene quantum dots as autophagy-inducing photodynamic agents[J]. Biomaterials, 2012,33(29):7084-7092.
[9]
Li P, Xu T, Wu S, et al. Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage in Caenorhabditis elegans[J]. J Appl Toxicol, 2017,37(10):1140-1150.
[10]
Wu C, Wang C, Han T, et al. Insight into the cellular internalization and cytotoxicity of graphene quantum dots[J]. Adv Healthc Mater, 2013,2(12):1613-1619.
[11]
Yuan X, Liu Z, Guo Z, et al. Cellular distribution and cytotoxicity of graphene quantum dots with different functional groups[J]. Nanoscale Res Lett, 2014, 9(1):108.
[12]
Eda G, Lin Y Y, Mattevi C, et al. Blue photoluminescence from chemically derived graphene oxide[J]. Adv Mater, 2010, 22(4):505.
[13]
Agarwal R, Singh V, Jurney P, et al. Mammalian cells preferentially internalize hydrogel nanodiscs over nanorods and use shape-specific uptake mechanisms[J]. Pnas, 2013,110(43):17247-17252.
[14]
Asati A, Santra S, Kaittanis C, et al. Surface-charge-dependent cell localization and cytotoxicity of cerium oxide nanoparticles[J]. Acs Nano, 2010,4(9):5321-5331.
[15]
Zhu J, Liao L, Zhu L, et al. Size-dependent cellular uptake efficiency, mechanism, and cytotoxicity of silica nanoparticles toward HeLa cells[J]. Talanta, 2013,107:408-415.
[16]
Soenen S J, Rivera-Gil P, Montenegro J M, et al. Cellular toxicity of inorganic nanoparticles: common aspects and guidelines for improved nanotoxicity evaluation[J]. Nano Today, 2011,6(5):446-465.
[17]
Thorek D L, Tsourkas A. Size, charge and concentration dependent uptake of iron oxide particles by non-phagocytic cells[J]. Biomaterials, 2008, 29(26): 3583-3590.
2018, Vol. 29 
