Effects of acid-elution temperature of hemoglobin F on precision of Kleihauer-Betke test
ZHU Hongyuan1, FAN Fei1, CAO Zheng1, CHEN Kexin2, ZENG Jiazi1, XIE Xin1, YANG Lijun3
1.Department of Clinical Laboratory,3.Develpoment and Operations Department, Obstetrics and Gynecology Hospital Affiliated to Capital Medical University, Beijing 100026, China; 2. Beijing Institute of Ophthalmology, Beijing Tongren Hospital, Beijing Tongren Eye Center, Capital Medical University, Beijing Ophthalmology & Visual Science Key Lab, Beijing 100730, China
Abstract：Objective To determine the optimum acid-elution temperature of Kleihauer-Betke (K-B) experiment for hemoglobin F detection and fetomaternal hemorrhage (FMH) evaluation.Methods Both 37 ℃ and 45 ℃ were chosen as the acid-elution temperatures in the K-B experiment, followed by head-to-head staining results comparison.Results When the elution temperature was set at 37 ℃ and specimens were negative, there was no statistically significant difference between the results of K-B experiments (< 1%) and theoretical values. However, with theoretically positive specimens (≥1%), the results of experiments were statistically different from the calculated values (P<0.05). When the elution temperature was set at 45 ℃, there was no significant difference between the K-B test results and their corresponding theoretical values, whether the specimens were positive or. negative.Conclusion When the elution temperature is set at 45 ℃ in the K-B experiment, we can more effectively and accurately distinguish the fetal red blood cells from maternal blood by the staining method. Our work provides helpful laboratory data support for the diagnosis, treatment and prognosis of FMH.
朱宏远, 樊斐, 曹正, 陈可心, 曾甲子, 谢鑫, 杨丽珺. F血红蛋白酸洗脱液温度对Kleihauer-Betke实验准确率的影响[J]. 武警医学, 2019, 30(5): 413-415.
ZHU Hongyuan, FAN Fei, CAO Zheng, CHEN Kexin, ZENG Jiazi, XIE Xin, YANG Lijun. Effects of acid-elution temperature of hemoglobin F on precision of Kleihauer-Betke test. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(5): 413-415.
Katherine M,Niyati M,Mara S. The role of Betke-Kleihauer testing in the detection of fetomaternal hemorrhage: a retrospective chart review[J]. JOGC, 2018, 40(6):843-844.
Kadooka M,Kato H,Kato A,et al. Effect of neonatal hemoglobin concentration on long-term outcome of infants affected by fetomaternal hemorrhage[J]. Early Hum Dev, 2014, 90(9): 431-434.
Mariela G F, Suzane D B, Simone M C, et al. Flow cytometry in detection of fetal red blood cells and maternal F cells to identify fetomaternal hemorrhage[J]. Fetal Pediatr Pathol, 2016, 35(6): 385-391.
[12]
Jennifer C D, Sharon M C, Aoife D. False positive Kleihauer-Betke (acid elution) test caused by elevated maternal fetal haemoglobin F cells[J]. Eur J Obstet Gynecol, 2014, 172: 136-137.
2019, Vol. 30 
