Human umbilical cord mesenchymal stem cells derived exosomes promote proliferation and differentiation of osteoblast progenitor cells
WANG Li1,2, DUAN Cuimi3, YUAN Fangfang1, WANG Yan3, GUO Ximin3, GUO Hongyan1,2
1.Department of Stomatology, the Third Medical Center of PLA General Hospital, Beijing 100039, China;
2.Graduate School of Jinzhou Medical University, Jinzhou 121001, China;
3.Beijing Military Medical Research Institute, Beijing 100850, China
Objective To extract human umbilical cord mesenchymal stem cells (hucMSCs) derived exosomes (hucMSC-exos) and observe their effects on mouse osteoblast progenitor cells (mOPCs).Methods HucMSCs were isolated and cultured in vitro. Exosomes were collected by ultracentrifugation from supernatants. After treatment with hucMSCs-exos at different concentrations, the proliferation of mOPCs was detected by CCK8 assay. Alkaline phosphatase activity (ALP) analysis and alizarin red staining were used to detect the effects of different concentrations of hucMSC-exo on the mineralization process of mOPCs.Results hucMSCs were derived that exhibited spindle-like morphology, vortex-shaped adherent growth and the potential for multidirectional induction of differentiation. HucMSC-exos were isolated that had spherical structures and a size of around 80nm. The results of Western blot analysis showed that hucMSC-exos expressed the exosomal markers CD9 and CD63. The Results of CCK8 showed that 5 μg/ml and 10 μg/ml hucMSC-exos could significantly promote the mOPC proliferation in vitro (P<0.05). The Results of alkaline phosphatase and alizarin red staining revealed the 5 μg/ml and 10 μg/ml hucMSC-exo group could significantly increase the alkaline phosphatase activity and calcified nodule formation of mOPCs. The role of 10 μg/ml exosomes was stronger than that of 5 μg/ml (P<0.05).Conclusions hucMSCs and hucMSC-exo have been isolated. hucMSC-exos can effectively promote the proliferation and osteogenic differentiation of mOPCs in a dose-dependent manner.
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2020, Vol. 31 
