Expression of COL6A2 and its clinical significance in gastric cancer
ZHENG Duoan1, ZHENG Manman2, MENG Xiangling3, SUN Dengqun1, SUN Yanjun1, CHEN Xiaoling4
1. Department of Laparoscopic Surgery,4. Department of Pathology,Anhui Provincial Corps Hospital of Chinese People’s Armed Police Force,Hefei 230041,China; 2. Department of Radiology,the First Hospital of Jiaxing, Jiaxing 314000, China; 3. Department of General Surgery, the First Affiliated Hospital of Anhui Medical University, Hefei 230022,China
Abstract：Objective To investigate the expression and clinical significance of type Ⅵ collagen alpha 2 chain (COL6A2) in gastric cancer, and to explore the possible underlying mechanisms. Methods Immunohistochemistry was used to detect the expressions of COL6A2 in sixty-two gastric cancer tissues and matched paracancerous tissues. The correlation between COL6A2 expressions and clinicopathological features was analyzed. The survival curve was plotted using the Kaplan-Meier method and validated by the Kaplan-Meier Plotter online database. Data about gastric cancer was downloaded from The Cancer Genome Atlas (TCGA) to analyze the correlation between COL6A2 and epithelial-mesenchymal transition-related protein expressions. Finally, Gene Set Enrichment Analysis (GSEA) was conducted to explore the possible mechanism of COL6A2 in gastric cancer. Results The positive expression rate of COL6A2 in gastric cancer tissues was significantly higher than in matched paracancerous tissues (43.5% vs. 25.8%, P=0.038). High expressions of COL6A2 were significantly correlated with lymph node metastasis, distant metastasis and TNM stage (P<0.05). Consistent with the result of Kaplan-Meier Plotter, survival analysis suggested that gastric cancer patients with high expressions of COL6A2 had a shorter survival time. Meanwhile, COL6A2 was an independent prognostic factor for gastric cancer (HR=2.339, 95% CI: 1.150-4.757, P=0.019). Correlation analysis revealed that COL6A2 expressions were closely related to the expressions of epithelial-mesenchymal transition-related proteins. GSEA showed that high expressions of COL6A2 samples were mainly enriched in several classical signaling pathways including TGF-beta signaling pathway and MAPK signaling pathway. Conclusions COL6A2 is highly expressed in gastric cancer tissues and is associated with poor prognosis. It could be a potential biomarker for the prognosis of gastric cancer patients.
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2020, Vol. 31 
