Effect of myeloid-derived growth factors on osteogenic differentiation of BMSCs in high glucose environment and its mechanism
ZHAO Xiqiang1, LU Wenhua1, SHANG Liqiang1, DANG Ruijie2, AN Ran1, HE Xin3
1. Department of Stomatology, the Third Medical Center of Chinese PLA General Hospital, Beijing 100039, China; 2. Medical Innovation Research Division, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; 3. Department of Stomatology, Rocket Force Characteristics Medical Center, Beijing 100088, China
Abstract：Objective To examine the effect of myeloid-derived growth factors (MYDGF) on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in high glucose environment and its molecular mechanism. Methods BMSCs were isolated and cultured in vitro, the surface markers of BMSCs were detected by flow cytometry, the osteogenic status of BMSCs was quantitatively detected by alkaline phosphatase (ALP), and the mRNA levels of OSX, RUNX2 and COL-I were detected by RT-qPCR. The protein levels of p-GSK3β and β-catenin were detected by western blot. Results ALP activity and mRNA expression of OSX, RUNX2 and COL-I in BMSCs were inhibited by high glucose (P＜0.05), and the inhibitory effect was improved after adding MYDGF (P＜0.05). The inhibitory effect of high glucose on the expression of p-GSK3β and β-catenin could be reversed by MYDGF [p-GSK3β:(0.85±0.13) vs. (0.15±0.07), β-catenin: (0.79±0.14) vs. (0.37±0.11); P＜0.05], and the promotion of MYDGF on the expression of p-GSK3β and β-catenin were inhibited after DKK1(the inhibitor of Wnt signaling pathway) treatment [p-GSK3β:(0.43±0.11) vs. (0.85±0.13), β-catenin:(0.20±0.06) vs. (0.79±0.14); P＜0.05]. Conclusions MYDGF may promote the osteogenic differentiation of BMSCs in high glucose environment by activating the Wnt/β-catenin signaling pathway.
赵喜强, 逯文华, 尚利强, 党瑞杰, 安然, 何鑫. 髓源性生长因子对高糖环境BMSCs成骨分化的影响及其机制[J]. 武警医学, 2023, 34(6): 507-510.
ZHAO Xiqiang, LU Wenhua, SHANG Liqiang, DANG Ruijie, AN Ran, HE Xin. Effect of myeloid-derived growth factors on osteogenic differentiation of BMSCs in high glucose environment and its mechanism. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(6): 507-510.
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