Abstract：Objective To investigate the relationship between serum microRNA(miR-155-5p) and miR-146b-5p and chromosome aberration and prognosis of secondary acute myeloid leukemia (sAML). Methods A total of 26 patients with sAML who were treated in the Department of Hematology of the Second the Second Hospital affiliated to Medical University of PLA Air Force from May 2017 to April 2020 were selected, and 25 healthy people who underwent physical examination in the hospital during the same period were included as healthy control group. According to International System for Human Cytogenetic Nomenclature, sAML patients were divided into chromosome aberrant group and chromosome normal group. The serum levels of miR-155-5p and miR-146b-5p were detected by real-time fluorescence quantitative PCR, and the overall survival of patients during follow-up was recorded. Results Compared with the healthy control group, serum miR-155-5p significantly increased[4.69(1.87,30.83) vs. 1.13(0.79,1.78), Z=-4.070,P＜0.001] and miR-146b-5p significantly decreased [1.79(1.55,2.44) vs. 3.02(2.58,3.43), Z=-4.561, P＜0.001]in the sAML group. The area under the curve of serum miR-155-5p and miR-146b-5p combined diagnosis of sAML was 0.922 (95%CI: 0.846~0.997).Compared with the normal group, the serum miR-155-5p significantly increased [19.26(4.45,46.65) vs. 1.51(0.79,3.27), Z=-3.585,P＜0.001]and miR-146b-5p significantly decreased [1.55(1.50,1.97) vs. 2.16(1.79,3.00), Z=-2.954, P=0.003]in the chromosomal aberration group. The AUC of serum miR-155-5p and miR-146b-5p levels to identify chromosome aberrations was 0.925 (95%CI: 0.826~1.000),0.850(95%CI: 0.703~0.997), and the combined AUC increased to 0.919(95%CI: 0.812~1.000). Multivariate Logistic regression analysis showed that serum miR-155-5p and miR-146b-5p levels were independent influencing factors of chromosome aberration (P＜0.05). Patients with high miR-155-5p expression (≥4.27) and low miR-146b-5p expression (＜1.62) had lower overall survival rate and shorter median survival time (P＜0.05). Cox regression analysis showed that high expression of miR-155-5p and low expression of miR-146b-5p in serum were independent risk factors for death in sAML patients (P＜0.05). Conclusions The high expression of miR-155-5p and the low expression of miR-146b-5p in serum are closely related to chromosome aberration and poor prognosis in sAML patients, and they may be risk predictors of chromosome aberration and poor prognosisof sAML patients.
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2024, Vol. 35 
