爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响

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摘要目的探讨爱帕琳肽13(Apelin-13)对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响。方法分离、培养GK糖尿病大鼠BMSCs。成骨诱导培养后,检测碱性磷酸酶(ALP)活性,Von Kossa染色观察钙结节矿化程度,O2K线粒体呼吸仪测定不同状态下线粒体呼吸功能。结果 Apelin-13增强GK糖尿病大鼠BMSCs的ALP活性;促进钙结节矿化;与GK组比较,Apelin-13有效提高两种状态下线粒体呼吸功能:复合物Ⅰ态3呼吸[(16.1±1.8) vs. (5.6±0.9)]pmol/(s×ml),复合物Ⅰ+复合物Ⅱ态3呼吸[(71.1±8.6) vs.(49.0±4.8)] pmol/(s×ml)。结论 Apelin-13可促进GK大鼠BMSCs骨向分化,并提高其线粒体呼吸功能。

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	李梦
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	张欣然
	朱彪
	郑雪
	王坚

关键词 ：爱帕琳肽13, 骨髓间充质干细胞, 线粒体呼吸

Abstract：Objective To investigate the effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats. Methods The BMSCs of GK diabetic rats were extracted. After cultured in osteogenic differentiation medium, the activity of alkaline phosphatase (ALP) was detected. Von Kossa staining was used to evaluate calcium nodules formation, and the mitochondrial respiratory function under different states was determined by O2K mitochondrial respiration apparatus. Results Apelin-13 enhanced the ALP activity of BMSCs in GK diabetic rats, and promoted calcium nodules mineralization. Compared with the GK group, Apelin-13 effectively improved mitochondrial respiratory function in two states: complex I state 3 respiration (16.1±1.8) vs. (5.6±0.9)] pmol/(s×ml) and complex Ⅰ+ complex Ⅱ state 3 respiration[(71.1±8.6) vs. (49.0±4.8)] pmol/(s×ml). Conclusions Apelin-13 can promote osteoblast differentiation and enhance mitochondrial respiration function of BMSCs derived from GK diabetic rats.

Key words： Apelin-13 BMSCs mitochondrial respiration

收稿日期: 2023-08-14

ZTFLH:

R780.2

基金资助:北京市医管局青年项目(QML20230809)

通讯作者: 王坚,E-mail:bunny_hi@126.com

作者简介: 李梦,本科学历,住院医师。

引用本文:

李梦, 李爽, 张欣然, 朱彪, 郑雪, 王坚. 爱帕琳肽13对GK大鼠BMSCs骨向分化及线粒体呼吸功能的影响[J]. 武警医学, 2024, 35(5): 395-398. LI Meng, LI Shuang, ZHANG Xinran, ZHU Biao, ZHENG Xue, WANG Jian. Effect of Apelin-13 on osteoblast differentiation and mitochondrial respiration function of BMSCs derived from GK diabetic rats. Med. J. Chin. Peop. Armed Poli. Forc., 2024, 35(5): 395-398.

链接本文:

http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2024/V35/I5/395

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