Effect of high-intensity interval training on mitochondrial autophagy in leukocyte after rush entry into plateau
LIU Jing1, ZHANG Hongying2, DUAN Fuqiang3, PENG Peng4, QIN Yongsheng4, and BO Hai4
1. Department of Pathology, Affiliated Hospital of Logistics University, Chinese People’s Armed Police Forces, Tianjin 300162, China; 2. Department of Quality Management, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China; 3. Department of Health, Xizang Autonomous Regional Corps Hospital, Chinese People’s Armed Police Forces, Lasa 850000, China; 4. Department of Military Training Medicines, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300309, China
Abstract Objective To explore the effect of short-term high-intensity interval training (HIT) in plain on mitochondrial autophagy in leukocyte after rush entry into plateau. Methods Thirty male lowlanders were randomly divided into two groups: untrained group (NT, n=15) and HIT group (n=15). Participants in the HIT group run at a high training intensity, 90%-95% heart rate reserve (HRR) for 3 min, with active pauses of 3 min of walking at 45%-50% of HRR. Participants in the HIT group underwent six sessions of HIT for 36 min/d, 7 days per week for 2 weeks. After training, participants in both HIT and NT groups traveled into plateau in the same batch. Activities of mitochondrial complex, PINK1, Bnip3 and Beclin-1 protein expression in leukocyte were measured in plateau at 24 h after entry. Results As compared with NT group, in HIT group, mitochondrial autophagy proteins Bnip3 (100±18 vs 136±32) and Beclin-1(100±19 vs 119±26) expression were significantly elevated(P<0.05). Furthermore, mitochondrial complex Ⅰ, Ⅱ, Ⅳ and ATP synthase activities were significantly elevated (P<0.05), whereas PINK1 protein expression was significantly decreased (P<0.01). Conclusions Short-term HIT in plain can blunt high altitude hypoxia induced mitochondrial impariment and elevated mitochondrial energy metabolism through upregulation of mitochondrial autophagy.
LIU Jing,ZHANG Hongying,DUAN Fuqiang等. Effect of high-intensity interval training on mitochondrial autophagy in leukocyte after rush entry into plateau[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2014, 25(12): 1228-1231.
Weng T P, Huang S C, Chuang Y F, et al. Effects of interval and continuous exercise training on CD4 lymphocyte apoptotic and autophagic responses to hypoxic stress in sedentary men[J].PLoS One, 2013,8(11):e80248.
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2014, Vol. 25 
