亚硒酸钠对海洛因吸食者T淋巴细胞增殖及其线粒体功能的影响

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Abstract Objective To study whether or not there is protective effect of sodium selenite on T lymphocytes of heroin addicts, and if it correlates with mitochondrial uncoupling related indicators. Methods T lymphocytes extracted from peripheral blood of people who abuse heroin in different periods. Cell proliferation was detected by MTT, the levels of ROS, △Ψm were detected by fluorescence spectrophotometer, MPTP was measured by ultraviolet spectrophotometer. Results With the increased duration of heroin-abuse, T lymphocyte proliferation activity decreased significantly(P<0.05); T lymphocyte mitochondrial MPTP increased, △Ψm decreased, ROS increased(P<0.05). Sodium selenite can reverse these changes, and there is a dose-effect relationship(P<0.05). Conclusions Oxidative stress caused by prolonged heroin abuse has an adverse effect on the function and proliferation of T lymphocytes via the mitochondrial pathway. Sodium selenite has a protective effect on T lymphocytes by its antioxidant effect, which reduces the level of ROS to reverse the above process.

Key words： sodium selenite T lymphocytes mitochondria mitochondrial permeability transition pore reactive oxygen species mitochondrial membrane potential

Received: 11 August 2014

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R392.12

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	Articles by authors
	FU Qingmei
	ZHANG Yongchun
	ZHANG Yongchuang
	BU Yingbo
	XIE Miao
	WANG Qian

Cite this article:

FU Qingmei,ZHANG Yongchun,ZHANG Yongchuang等. Effects of sodium selenite on T lymphocyte proliferation and mitochondrial function in vitro[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(2): 175-179.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I2/175

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