胰岛素对链脲佐菌素诱导的糖尿病大鼠<br/>骨转换和骨力学性能的影响

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Abstract Objective To study the effect of insulin on bone mass and the condition of bone turnover and bone biomechanical properties in STZ-induced diabetic rats. Methods Of 45 male SD rats studied, 30 were made diabetic by intravenous injection of streptozocin(50 mg/kg) on day -7. Thirty diabetic rats were randomly divided into 2 groups as group DM and control group. DM group were injected subcutaneously with insulin (6 U/kg/d) regularly.After their sacrifice on the day 56, blood was sampled and serum was separated for the measurement of Ca, P, ALPand OC. The left tibia was dissected for bone histomorphometry analysis. Right femur and lumbar vertebrae(L1-L4) were reserved for BMD test. The left femor was separated for three-point bending test,while the fifth lumbar vertibra was prepared for compression test to reflect the biomechanical properties of bone.Results A low-turnover osteopenia was evidenced in diabetic rats by decreased BMD in both femur and lumbar vertebrae, reduced serum OC level, decreased urine NTx/Cr ratio, decreased trabecular volume[TBV/TTV(12.06±2.48) vs (24.12±1.84)%, P＜0.01]and bone formation rate[(0.44±0.11) vs (0.78±0.14)μm/d, P＜0.01] by bone dynamic study. All these abnormalities except urine NTx/Cr ratio were partly or completely normalized by insulin treatment. The bone densities in vertebrae and femur of diabetic rats significantly decreased(P＜0.01). Maximal load, elastic load, bending energy, maximal stress in three-point bending test were conducted,compared with normal control rats. These abnormalities could be partially corrected by insulin teatment(P＜0.01).In addition,the fifth lumbar vertibrae of diabetic rats were obviously decreased(P＜0.01), and maximal load,elastic load,energy absorbability and elastic modulus during compressing test. By insulin treatment, these indices partially reversed to the normal control level(P＜0.01). Conclusion A low-turnover osteopenia is evidenced in STZ-induced diabetic rats with impaired bone biomechanical abilities.All these abnormalities of bone can be partially corrected by the injection of insulin. It shows that insulin can stimulate bone formation dramatically while not influence osteoclastic activity in diabetic rats.

Key words： diabetic mellitus insulin bone mineral density bone histomorphemetry bone biomechanical test

Received: 03 March 2015

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	Articles by authors
	LIN Huagang
	WANG Bo
	ZHANG Jun
	and JIA Hongwei

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LIN Huagang,WANG Bo,ZHANG Jun等. Effects of insulin on diabetic bone histomorphometric and biomechanical abilities in streptozotocin-treated rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(10): 1010-1014.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I10/1010

[1]	White C B, Turner N S, Lee G C, et al. Open ankle fractures in patients with diabetes mellitus[J]. Clin Orthop Relat Res, 2003, 41(4):37-44.
[2]	Thrailkill K M, Liu L, Wahl E C,et al. Bone formation is impaired in a model of type 1 diabetes[J]. Diabetes,2005,54(10):2875-2881.
[3]	Gandhi A, Beam H A, O’Connor J P,et al.The effects of local insulin delivery on diabetic fracture healing[J]. Bone,2005,37(4):482-490.
[1]	White C B, Turner N S, Lee G C, et al. Open ankle fractures in patients with diabetes mellitus[J]. Clin Orthop Relat Res, 2003, 41(4):37-44.
[4]	Margonar R, Sakakura C E, Holzhausen M,et al. The influence of diabetes mellitus and insulin therapy on biomechanical retention around dental implants: a study in rabbits[J]. Implant Dent,2003,12(4):333-339.
[2]	Thrailkill K M, Liu L, Wahl E C,et al. Bone formation is impaired in a model of type 1 diabetes[J]. Diabetes,2005,54(10):2875-2881.
[3]	Gandhi A, Beam H A, O’Connor J P,et al.The effects of local insulin delivery on diabetic fracture healing[J]. Bone,2005,37(4):482-490.
[4]	Margonar R, Sakakura C E, Holzhausen M,et al. The influence of diabetes mellitus and insulin therapy on biomechanical retention around dental implants: a study in rabbits[J]. Implant Dent,2003,12(4):333-339.
[5]	孙丽莎,刘润萍,陈秋.糖尿病合并骨质疏松症的发病机制和治疗[J].山东医药,2012,52(17):92-97.
[6]	王燕,孟广军.60例2型糖尿病患者的骨密度分析[J].中国临床实用医学,2009,3(9):98-99.
[7]	梁军波,徐春丽,潘伟波,等.高血糖对成骨细胞增殖分化影响的实验研究[J].医学研究杂志,2012,41(3):117-121.
[5]	孙丽莎,刘润萍,陈秋.糖尿病合并骨质疏松症的发病机制和治疗[J].山东医药,2012,52(17):92-97.
[6]	王燕,孟广军.60例2型糖尿病患者的骨密度分析[J].中国临床实用医学,2009,3(9):98-99.
[7]	梁军波,徐春丽,潘伟波,等.高血糖对成骨细胞增殖分化影响的实验研究[J].医学研究杂志,2012,41(3):117-121.
[8]	沈静雪,田丰,刘英敏,等.肿瘤坏死因子α和白介素6在2型糖尿病发病及骨密度改变中的作用[J].中国实用内科杂志,2003,23(9):535-537.
[8]	沈静雪,田丰,刘英敏,等.肿瘤坏死因子α和白介素6在2型糖尿病发病及骨密度改变中的作用[J].中国实用内科杂志,2003,23(9):535-537.
[9]	Pun K K, Lau P, Ho P W. The characterization, regulation and function of insulin receptors on osteoblast-like clonal osteosarcoma cell line[J] Bone Miner Res, 1989, 4(6):853-862.
[9]	Pun K K, Lau P, Ho P W. The characterization, regulation and function of insulin receptors on osteoblast-like clonal osteosarcoma cell line[J] Bone Miner Res, 1989, 4(6):853-862.