缬沙坦抑制内质网应激对糖尿病肾损害大鼠足细胞的保护作用

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Abstract Objective To evaluate the effect of valsartan on apoptosis of podocytes,GRP78 which serve as a symbolic protein of endoplasmic reticulum stress (ERS) and caspase12 in diabetic rats. Methods Male SD rats were randomly divided into normal group and study group.The animal models with diabetes were established in study group by STZ intraperitoneal injection,then the study group were randomly divided into model group and valsartan group.Water was aiministered daily by gavage to normal group and model group.Valsartan was administered daily by gavage to valsartan group for 6 weeks. The levels of protein of nephrin、GRP78,caspase 12.BG,Cr ,BUN and 24-hour urinary protein quantity were checked. Results At the time of 6 weeks,compared with those in normal group,the number of apoptosis cells were much higher in the model kidneys,the expression of GRP78(6.75±0.65),caspase 12 (11.51±0.32)in the model kidneys were much higher than GRP78(1.57±0.39),caspase12(2.66±0.57)in the normal group(P＜0.05);however, the expression of nephrin in the model group(2.29±0.15)was lower than in the normal group(5.71±0.53)(P＜0.05). While in the valsartan group,reductions were found in the expression of GRP78(3.14±1.00),caspase12 (8.08±2.48)and the number of apoptotic cells(P＜0.05),the expression of nephrin (3.35±0.40)was higher(P＜0.05). Conclusions Valsartan can depress the effect of endoplasmic reticulum stress and reduce the apotosis of the podocytes in the development of diabetic nephropathy.

Key words： diabetic nephropathy endoplasmic reticulum stress apoptosis nephrin valsartan

Received: 08 May 2015

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