罗格列酮对重症急性胰腺炎大鼠胰腺氧化应激的影响

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Abstract Objective To investigate the effect of rosiglitazone on oxidative stress in rats with severe acute pancreatitis.Methods Sixty male SD rats were randomly divided into 3 group(n=20): sham operation group (SO group), severe acute pancreatitis group(SAP group) and rosiglitazone treatment group (ROSI group). SAP model was induced by retrograde infusion of 5% sodium taurocholate into the biliopancreatic duct. The rats in ROSI group were injected rosiglitazone(6 mg/kg) throngh femoral vein 5 minutes after the operation. In SO group and SAP group saline partes equales were injected. Rats were killed at 12 h after operation. Analysis of the mortality of rats in each group was conducted. Detecting amylase(AMY), nitric oxide(NO), malondialdehyde(MDA)、glutathione peroxidase(GSH-Px), pancreas pathologic score. Enzyme-linked immunosorbent assay (ELISA)and analysis of serum interleukine-1β (IL-1β), interleukine-6 (IL-6) levels were carried out. The expression of inducible nitric oxide synthase(iNOS)、endothelial nitric oxide synthase(eNOS)in pancreas tissue were measured by Western blotting method.Results Two rats died in SAP group, no rat died in SO group and ROSI group. Compared with SO group, the levels of AMY, NO, MDA and pancreas pathologic score were significantly increased in SAP group.The level of GSH-Px activity decreased significantly in SAP group than in SO group[(39.93±15.18) vs (59.81±22.43) U/mg], which was higher in ROSI group than in SAP group[(49.01±9.42) vs (59.81±22.43) U/mg] (P<0.05). The expression of iNOS and eNOS in pancreas tissue increased significantly in SAP group than in SO group[(1.90±0.16) vs (0.75±0.09),(0.37±0.09) vs (0.09±0.01)] (P<0.05); compared with SAP group, these indexes decreased significantly in ROSI group[(1.31±0.18) vs (1.90±0.16)、(0.23±0.08) vs (0.37±0.09)] (P<0.05).Conclusions Rosiglitazone exerts the protective effect on severe acute pancreatitis through inhibiting oxidative stress and downregulating the expression of inflammatory cytokine.

Key words： rosiglitazone pancreatitis oxidative stress inflammatory cytokine

Received: 11 April 2016

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	Articles by authors
	CHEN Chen
	GUO Wenyi
	YU Jia
	ZHAO Kailiang
	and WANG Weixing

Cite this article:

CHEN Chen,GUO Wenyi,YU Jia等. Effect of rosiglitazone on oxidative stress in rats with severe acute pancreatitis[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(8): 767-770.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I8/767

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