模拟失重雌雄大鼠骨密度、骨代谢指标与骨折风险的相关性

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Abstract Objective To analyze the changes of BMD, biochemical marker parameters, mechanical properties in male and female rat osteoporosis models in simulated weightlessness comparatively to establish relationship and predict fracture risk.Methods 40 3-months-old SD rats were selected, 20 males and 20 females. According to the gender, they were divided into 4 groups at random: experimental group and reference group. The t Tail suspended models (TS) were measured. After 4 weeks, the rats were killed, the BMD of L₄ and femoral condyle by dual-energy X-ray absorptiometry (DEXA) were measured, the osteal tissue slices were stained by the way of poncean, the marks of bone biochemical metabolism in serum were detected by ELISA and the biomechanics was tested by biomechanical testing machine.Results The BMD of L₄ lumbar vertebrae and femoral condyle in the experimental groups are obviously lower than the reference groups. The bone metabolic biochemical markers (BLAP & TRAP) in TS groups were remarkably higher than in the reference groups. The correlation coefficient R values between Fmax and BMD, BLAP, TRAP in male groups were 0.985, －0.949, －0.970, and in female group were 0.908, －0.858, －0.921.Conclusions For 4 weeks simulated weightlessness, obvious osteoporosis, destruction of the microstructure, decrease of the mechanical strength are manifested. Fmax is positively correlated with BMD, and negative correlation for BLAP, TRAP. It is a precise and convenient bone metabolism index to predict fracture risk in space medicine and is a feasible, precise and convenient bone metabolism index to predict fracture risk in space medicine.

Key words： simulated weightlessness osteoporosis biochemical marker biomechanics correlation

Received: 03 November 2015

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	Articles by authors
	ZHANG Heng
	REN Ningtao
	LIU Ning
	LI Jie
	WANG Zheng
	MAO Keya
	and CUI Geng

Cite this article:

ZHANG Heng,REN Ningtao,LIU Ning等. The relationship between BMD, biochemical marker parameters and fracture risk for simulated weightlessness in male and female rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(6): 545-549.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I6/545

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