血管内皮祖细胞对骨质疏松大鼠骨髓间充质干细胞脂肪化的影响

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Abstract Objective To investigate the effect of endothelial progenitor cells (EPCs) on adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) of osteoporosis rats in order to provide a new strategy for prevention and treatment of osteoporosis.Methods An osteoporosis animal model was established with six-week-old female SD rats. EPCs and BMSCs of these rats were cultivated and identified in vitro. BMSCs at passage 3 and 6 were used respectively to establish a BMSC-EPC indirect co-culture system on transwell inserts. The experimental groups included the BMSC sham group, BMSC ovx group and BMSC ovx/EPC indirect co-culture group. MTT assay was applied to measure the cellular proliferation while Oil Red O solution was used to detect lipid droplets within the cells. Real-time PCR analyses were performed to analyze the expression of adipogenic marker PPARγ2.Results Under indirect co-culture conditions, EPCs could significantly promote the proliferation of BMSCs(ovx) and greatly inhibit the cells’capacity of self-adipogenic differentiation. Co-cultured EPCs resulted in a better proliferative ability of BMSCs in the BMSC ovx/EPC group than in the BMSC ovx group. Furthermore, BMSCs in the BMSC ovx/EPC group had a lower self-adipogenic differentiation potential than in the BMSC ovx group, which was evidenced by Oil Red O staining quantification and mRNA expression levels of adipogenic marker gene PPARg2.Conclusions Under indirect co-culture conditions, EPCs can enhance the stem cells’ proliferation but notably inhibit autonomic adipogenic differentiation of BMSCs of osteoporosis rats.

Key words： endothelial progenitor cell mesenchymal stem cell osteoporosis cell proliferation adipogenic differentiation

Received: 10 March 2017

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R329.28

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	Articles by authors
	WEN Li
	CHANG Ping
	CAI Chuan
	and LI Rui

Cite this article:

WEN Li,CHANG Ping,CAI Chuan等. Effect of endothelial progenitor cells on adipogenic differentiation of bone marrow mesenchymal stem cells of osteoporosis rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(8): 804-808.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I8/804

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