探索miRNA-155对急性髓系白血病患者预后影响及可能机制

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Abstract Objective To investigate the effect of miRNA-155 on prognosis of acute myeloid leukemia (AML) patients and its possible mechanism.Methods The miRNA and RNA expression profiles and clinical data of patients with AML in the TCGA database were downloaded. According to the expression level of miRNA-155, the first 50% were assigned to the high expression group (n=81), while the rest were assigned to the low expression group (n=81). The survival time of the two groups was compared. Pearson correlation analysis was used to detect the expression level of each RNA and miRNA-155, and the top 50 genes either positively or negatively correlated with miRNA-155 were selected according to the correlation coefficients. Analysis of the KEGG pathway and GO enrichment, as well as the protein interaction networks, were completed by the STRING tool.Results The median survival time in the low expression group was longer than that of the high expression group (609 d vs 485 d,P<0.05). Among the 19448 genes detected, the positive correlation coefficient of chromosome 21 open reading frame 71(C21orf71) was the highest(r=0.634,P<0.05), so was the negative correlation coefficient of solute carrier family 13 member 5 (SLC13A5) (r=-0.50,P<0.05).The GO enrichment analysis and the KEGG pathway enrichment analysis showed that the main biological processes involved were DNA replication initiation and DNA strand extension, the main molecular functions involved were the activity of helicase and the catalytic activity of DNA, the main cell components involved were microchromosome maintenance complex and tertiary granules, and the main signaling pathways involved were DNA replication and cell cycle regulation.The microchromosome maintenance protein(MCM) gene family was at the core of the protein interaction network.Conclusions Overexpression of miRNA-155 is associated with decreased long-term survival, which may be related to its regulation of cellular DNA replication.

Key words： acute myeloid leukemia miRNA survival curve bioinformatics

Received: 20 February 2019

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	Articles by authors
	SHEN Rui
	LI Jieping
	ZHAN Huien
	PENG Jianjiao
	XU Hongxiang
	ZENG Huiqi

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SHEN Rui,LI Jieping,ZHAN Huien等. Effect of miRNA-155 on prognosis of acute myeloid leukemia patients and its possible mechanism[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(11): 967-971.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2019/V30/I11/967

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