HPLC法同时测定参龙通络丸中三七皂苷R<sub>1</sub>和人参皂苷Rf的含量

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Abstract Objective To establish an HPLC method for simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills.Methods High performance liquid chromatography was used.Hypersil Hypersil BDS C₁₈(5 μm,4 mm×250 mm) column was selected with acetonitrile-water as the mobile phase for gradient elution.The flow rate was 1.0 ml/min,detection wavelength was 203 nm and the column temperature was set at 35 ℃.Results The linear ranges of Notoginsenoside R₁ and ginsenoside Rf were 0.198-9.535 μg (r=0.9993) and 0.957-38.392 μg(r=0.9997) respectively.The average recoveries were 99.10％(RSD=1.57％,n=6) and 98.76％(RSD=2.13％,n=6)respectively.Conclusions This HPLC method was accurate,reliable and reproducible,which can contribute to formulating the quality standard of Shenlong Tongluo pills.

Key words： Shenlong Tongluo pills Notoginsenoside R₁ ginsenoside Rf HPLC

Received: 20 September 2020

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	Articles by authors
	YANG Yuanyuan
	LUAN Tao
	SUN Yukun
	GUO Zhaogang

Cite this article:

YANG Yuanyuan,LUAN Tao,SUN Yukun等. Simultaneous determination of Notoginsenoside R₁ and ginsenoside Rf in Shenlong Tongluo pills by HPLC[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(1): 15-18.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I1/15

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