菊粉调控 NF-κB 信号抑制细胞凋亡减轻小鼠炎症性肠病的研究

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Abstract Objective To explore the effect and mechanism of inulin on intestinal inflammation in mice with inflammatory bowel disease (IBD). Methods Forty C57BL/6 mice were randomly divided into normal control group, inulin control group, model group and inulin treatment group (n=10). The inulin control group and inulin treatment group were gavaged 500 mg/kg inulin daily for 14 days, while the normal control group and model group were gavaged 0.9% Sodium Chloride Injection daily. The model group and inulin treatment group were induced IBD models by drinking 2.5% DSS for 7 days on day 8, while the normal control group and the inulin control group were given distilled water. The mice were killed 14 days later. The general condition and disease activity index (DAI) scores were analyzed, the colon length was measured, and the pathological changes of colon tissues were compared. The mRNA expression of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and interleukin-6(IL-6)in colon tissues were detected by real-time fluorescent quantitative PCR, and the expression of apoptosis-related proteins and NF-κB proteins in colon tissues was detected by western blot. Results Compared with the normal control group, the model group was generally worse, DAI score increased (P<0.05), colon length was shortened [ (6.61±0.33) cm vs. (8.21±0.32) cm, P<0.05], mRNA expressions of TNF-α, IL-1β and IL-6 increased (P<0.05), Bcl-2 protein decreased, Bax, cleaved caspase-3 and NF-κB protein increased (P<0.05), but there was no significant difference in the inulin control group. Compared with model group, the inulin treatment group was significantly improved in general condition, DAI score decreased (P<0.05), colon length recovered [(7.06±0.31) cm vs. (6.61±0.33) cm, P<0.05], mRNA expression of TNF-α, IL-1β and IL-6 decreased (P<0.05), Bcl-2 expression increased, and Bax and cleaved caspase-3 expression decreased (P<0.05). Conclusions Inulin can inhibit intestinal inflammation and cell apoptosis by inhibiting NF-κB , thereby improving the symptoms of IBD mice.

Key words： inulin inflammatory bowel disease NF-κB cell apoptosis

Received: 28 August 2023

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	Articles by authors
	WANG Ruilan
	WANG Yuexing
	YANG Jian
	andDENG Lijuan

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WANG Ruilan,WANG Yuexing,YANG Jian等. Inhibition of cell apoptosis and alleviation of intestinal inflammation in mice with inflammatory bowel disease through regulating NF-κB signaling by inulin[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(10): 874-879.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I10/874

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