吡非尼酮对大鼠坐骨神经损伤后神经再生和功能恢复的作用

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Abstract Objective To investigate the effect of pirfenidone on nerve regeneration in rats with sciatic nerve injury. Methods Rats with sciatic nerve injury were divided into four groups: normal saline group (control group, Ctrl), 25 mg/kg pirfenidone group (low-dose group, LG), 50 mg/kg pirfenidone group (medium-dose group, MG), and 100 mg/kg pirfenidone group (high-dose group, HG). An autograft repair model was established. The morphology of regenerative nerves was evaluated by electron microscopy (TEM). The recovery of motor function was assessed by Masson staining and sciatic functional index (SFI). The levels of transforming growth factor-β₁ (TGF-β₁) in regenerative nerves were determined by immunohistochemical staining. Results TEM results showed that the diameter of myelinated nerve fibers and the mean thickness of myelin sheath in the MG and HG groups were higher than those in the Ctrl and LG groups, and the G ratio were lower than those in the Ctrl and LG groups at 12 weeks after surgery. At both 8 and 12 weeks after surgery, the value of the percentage of gastrocnemius fibers and SFI in the MG and HG groups were significantly higher than those in the Ctrl and LG groups, and the differences were statistically significant(P<0.05). Immunohistochemical results indicated that TGF-β₁ expression in the MG and HG groups was significantly down-regulated compared with the Ctrl and LG groups, and the difference was statistically significant (P<0.05). Conclusions Pirfenidone can promote nerve regeneration and improve muscular function in rats with peripheral nerve injury, which may be related to its anti-fibrotic properties of pirfenidone.

Key words： pirfenidone peripheral nerve injury nerve scarring nerve regeneration

Received: 29 September 2024

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	QI Jingtian
	ZHANG Yongguang
	XU Yongjun
	SHENG Qingsong
	CAI Longyu
	YE Yongping

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QI Jingtian,ZHANG Yongguang,XU Yongjun等. Effects of pirfenidone on nerve regeneration and functional recovery after sciatic nerve injury in rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2025, 36(2): 124-128.

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https://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR https://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2025/V36/I2/124

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