Effect of 5-fluorouracil-loaded carbon nanoparticles on apoptosis of cells in gastric carcinoma and lymph node metastasis
CHEN Guo1,LI Ang2,DU Ling1,and LIU Xian Jun1.
1. Department of General Surgery,Sichuan Provincial Corps Chengdu Hospital,Chinese People’s Armed Police Forces,Chengdu 610041,China；2. Department of General Surgery,West China Hospital of Sichuan University, Chengdu 610041,China
Abstract：Objective To investigate the efficacy of 5-fluorouracil-loaded carbon nanoparticles, the targeted chemotherapeutic drug, on apoptosis index of cells in metastatic lymph nodes, gastric cancer and the normal gastric tissues. Methods Thirty-six patients with gastric cancer in this department were divided into lymph node-targeted chemotherapy group (n=18) and control group (n=18). The chemotherapy group had operation after the lymphatic target chemotherapy with 5-fluorouracil-loaded carbon nanoparticles. However, in control group, the patients were operated directly. The apoptosis index of cells in gastric carcinoma tissues, metastatic lymph nodes, and the normal gastric tissues after the operation were detected by TUNEL. Results In the lymph node-targeted chemotherapy group, apoptosis index（AI） of cells in gastric carcinoma tissues and metastatic lymph nodes were （17.30±3.50）% and （32.64±4.72）%, respectively, however, in the control group these were （4.81±2.76）% and （7.03±2.15）%, respectively. Consequently, the apoptosis index of cells in gastric carcinoma tissues and metastatic lymph nodes in the lymph node-targeted chemotherapy group was higher than that in the control group (P<0.05). Meanwhile, in the lymph node-targeted chemotherapy group, the AI of cells in normal gastric tissues was （13.73±3.60）%; however in the control group, the apoptosis index of cells in nomal gastric tissues was （14.10±3.87）% . There was no significant difference in the apoptosis index between the two groups (P>0.05). Conclusions 5-Fluorouracil-loaded carbon nanoparticles can target gastric carcinoma tissues and metastatic lymph nodes, therefore, increasing the apoptosis index which can promote tumor cell death. However, it has no obvious effect on the apoptosis index in normal gastric tissues.
2013, Vol. 24 
