Abstract：Objective To study the alteration of SAX2 protein expression pattern in rat serum and hippocampus after closed head injury and to find biomarkers of brain injury. Methods Male Sprague-Dawley rats were randomly divided into sham operation group and injury groups which were subjected to Marmarou’s brain injury and then subdivided into 4 h、8 h、12 h、24 h and 48 h groups according to the time elapsed after injury. The pathological study included H&E staining and toluidine blue staining. The change of protein expression pattern in serum and hippocampus were monitored with strong anion exchange chips (SAX2). Results (1)A total of 530 protein peaks were detected on SAX2 chips in serum,and a total of 147 protein peaks in hippocampus. (2) Among the total of protein peaks detected, the relative intensity of 45 protein peaks was shown to be differentially altered on SAX2 chips in serum of injury groups, compared with sham operation group(P＜0.05). There were 97 protein peaks in serum of injury groups, which could not be detected in sham operation group with SAX2 chips. (3) The relative intensity of 18 protein peaks was shown to be differentially altered on SAX2 chips in hippocampus of injury groups compared with sham operation group(P＜0.05). There were 40 protein peaks in hippocampus of injury groups which could not be detected in sham operation group with SAX2 chips. (4) On SAX2 chips, only one protein(3656Da) peak was detected not only in serum but also in hippocampus. Conclusions The alteration of SAX2 protein expression patterns in serum and hippocampus can be induced after brain injury. The SAX2 protein expression patterns of serum and hippocampus after brain injury are different, which suggests that part of protein in serum can be a production of blood cells gene expression induced by brain injury. The distinct proteins detected in serum and hippocampus includ the proteins which are significant, compared with sham operation group and those which are not detected in sham operation group. They can be biomarkers of brain injury.
赵彦功,舒清明,程芮,白皛,罗敏,段媛媛. 闭合性颅脑损伤大鼠血清和海马SAX2蛋白质谱的表达[J]. 武警医学, 2015, 26(11): 1087-1090.
ZHAO Yangong,SHU Qingming,CHENG Rui,BAI Xiao,LUO Min,and DUAN Yuanyuan. Alteration of SAX2 protein expression pattern in rat serum and hippocampus after closed head injury. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(11): 1087-1090.
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2015, Vol. 26 
