Effect of osteopontin on differentiation of osteoclasts induced by RANKL
WANG Zengtian1, XING Jie2, and LI Tan2
1. Department of Clinical Medicine, 2. Department of Pathogen Biology and Immunology, Logistics University of the Chinese People’s Armed Police Force, Tianjin 300162, China
Abstract：Objective To study the effect of osteopontin (OPN) on differentiation of osteoclasts (OC) by RANKL in vitro. Methods Mouse osteoclasts precursor cell line RAW264.7 was cultured and induced to form OC, and was added with osteopontin (OPN). Then, the proliferation and differentiation of OC were detected by MTT assay and TRAP staining. Furthermore, RT-PCR was used to measure the gene expression of RANK. Results The model of OC in vitro was established successfully, accompanied by the significant increase of proliferation and differentiation of OC (P<0.05). Meanwhile, OPN promoted the expression of RANK mRNA in OC obviously, which could be increased 2.67 times. Conclusions OPN affects the differentiation of RAW264.7 cells to form OC via RANK/ RANKL pathway, which may provide a new target for the prevention and treatment of osteoporosis and bone-related diseases.
王增田,邢杰,李覃. 骨桥蛋白对RANKL诱导破骨细胞分化的影响[J]. 武警医学, 2015, 26(12): 1221-1223.
WANG Zengtian, XING Jie, and LI Tan. Effect of osteopontin on differentiation of osteoclasts induced by RANKL. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(12): 1221-1223.
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2015, Vol. 26 
