Protective effect and mechanism of amifostine on intestinal acute radiation sickness
ZHAO Zhiwei1,2, CUI Yu2,WANG Limei2, SHAN Yajun2,TANG Hongwei3, CONG Yuwen2
1.Graduate School,Xuzhou Medical College, Xuzhou 221000, China; 2.Institute of Radiation Medicine,Academy of Military Medical Science,Beijing 100850,China; 3.Department of Gastroenterology,The General Hospital of Chinese People’s Armed Police Forces, Beijing100039,China
Abstract：Objective To study the radioprotection effect and mechanism of amifostine (WR2721) on intestinal acute radiation sickness. Methods Based on the intestinal acute radiation sickness model, C57BL/6J mice were randomly divided into control group and group WR2721. Bone marrow cells from healthy mice were transplanted after 15 Gy 60Co-γ shielded body irradiation. Then the survival rate in 30 d after irradiation, and the number of proliferative crypt cells and the small intestine mucosa situation with micro-cloning survival assay of mouse crypt cells at 3.5d post-irradiation were observed . Results The mice in control group died within 10 days, survival rate in WR2721 administrered group increased to 80%; in the control group and WR2721 drug group, the average number of crypts were 6.1±0.7, 28±1.1, the difference was statistically significant (P<0.0001); in control group and WR2721 administered group, villus heights were(51.48±2.04)μm, (73.29±3.24)μm, the difference was statistically significant (P<0.0001). Conclusions WR2721 has significantly radioprotective effect on intestinal acute radiation sickness by improving the survival rate of mice, promoting crypt cells regeneration and protecting the intestinal mucosa.
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2015, Vol. 26 
