Transplantation of autologous bone marrow mesenchymal stem cells into neural stem cells for treatment of cerebral palsy: 2-year follow-up of 11 cases
WANG Yali1,QI Jiangtong1, ZHANG Jinlong1,MA Yuhai1,and LIU Houqi2
1. Zhejiang Provincial Corps Hospital, Chinese People’s Armed Police Force,Jiaxin 314000,China; 2. Institute of Developmental Biology, Second Military Medical University, Shanghai 200433,China
Abstract：Objective To study the efficacy of autologous bone marrow mesenchymal stem cells (human mesenchymal stem cells, hMSCs) transplanted into neural stem cells for the treatment of cerebral palsy. Methods The transplantation group included 11 children with cerebral palsy; another 11 children with cerebral palsy and of the same age, gender and disease severity was as control group. The transplantation group received transplantation of autologous bone marrow mesenchymal stem cells into neural stem cells for the treatment of motor function and exercise-based rehabilitation, where as the control group were only given exercise-based rehabilitation. Both groups underwent gross motor function assessment (GMFM) before treatment and 1 month, 3 months, 6 months, 12 months, 18 months, and 24 months after treatment. Results (1) In the transplantation group 1 month, 3 months, 6 months after treatment,GMFM0 was significantly higher than that in the control group (P<0.025). In transplantation group 3 months after therapy, the rising rate of GMFM3 over GMFM1 had significant difference; and 6 months after treatment the rising rate of GMFM3 over GMFM6 after three months had no statistically significant difference. (2) Follow-up of 12 months, 18 months and 24 months found that between the transplantation group and the control group GMFM12, GMFM18, and GMFM24 had no siqnificant difference in the rate of rise, whereas compared the GMFM the rising rate in with the six preceding months, had significant difference ( P<0.01). (3) 1 month,3 months after the treatment in the two groups, language quotients rising rate compared with pre-treatment language quotients rising rate had no significant difference. 6 months and 12 months after treatment, in transplantation group, rise rate of language quotients was significantly higher than pre-treatment (P<0.01) and 18 months and 24-month follow-up showed that the rise rates of language guofients were not apparently faster than the control, but faster than pre-treatment with a significant difference (P<0.05). Conclusions Transplantation of autologous bone marrow mesenchymal stem cells into neural stem cells for the treatment of children with cerebral palsy is effective and safe, markedly improves motor function, 3 months after transplantation. The exercise rehabilitation effects gradually disappear after 6 months, but the motor ability in children had no retrogradation. Marked improvement of language function is late in the 6 months after transplantation and marked rapid improvement of language disappears after 12 months, but there is no language skills setback.
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2016, Vol. 27 
