Simultaneous determination of ferulic acid and glycyrrhizic acid in Xiao Yao Wan by HPLC
TIAN Jie1, SUN Yukun2, YIN Lu2, TANG Zhe2, and WANG Haipeng2
1.Department of Pharmacy,Chinese Medicine Hospital of Tangshan,Tangshan 063000, China; 2. Department of Pharmacy,Liaoning Provincial Crops Hospital,Chinese People’s Armed Police Force,Shenyang 110034,China
Abstract：Objective To establish a method for the determination of ferulic acid and glycyrrhizic acid in Xiao Yao Wan.Methods The separation was performed on an Agilent Zorbax XDB-C18 column(150 mm×4.6 mm,5 μm)with acetonitrile/ phosphoric acid solution(0.05%) as the mobile phase with gradient elution at the flow rate of 1.0 ml/min. The detection wavelength was set at 278 nm, and the column temperature was 35 ℃.Results The linear range of ferulic acid and glycyrrhizic acid was 0.054-0.596 μg (r=0.9995) and 0.110-1.217 μg (r=0.9993) respectively. The average recovery was 99.29％(RSD=1.28％, n=6) and 98.34％(RSD=1.61％,n=6) respectively.Conclusions The established HPLC method has proved to be user-friendly, reproducible, precise and suitable for the content determination of Xiao Yao Wan.
田洁, 孙玉坤, 尹禄, 唐哲, 王海鹏. HPLC法同时测定逍遥丸中阿魏酸和甘草酸含量探讨[J]. 武警医学, 2019, 30(10): 845-848.
TIAN Jie, SUN Yukun, YIN Lu, TANG Zhe, and WANG Haipeng. Simultaneous determination of ferulic acid and glycyrrhizic acid in Xiao Yao Wan by HPLC. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(10): 845-848.
2019, Vol. 30 
