Effects of irisin on adipogenesis of bone-marrow mesenchymal stem cells from rabbits
ZHAI Yu1, DONG Keming1, GUO Hongyan1, LIU Cui1, XU Hongmei1, SHEN Yechun2, LI Jinru3, MEI Wen4
1. Department of Stomatology, the Third Medical Center of Chinese PLA General Hospital, Beijing 100039,China; 2. Department of Stomatology, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, China; 3. Department of Stomatology, Hong Kong Garrison Hospital of Chinese PLA, Hong Kong 999077, China; 4. Department of Endocrinology, People's Hospital of Nanhai District, Foshan 528200, China
Abstract：Objective To investigate the effects of irisin on adipocyte differentiation of BMSCs from rabbits and related mechanisms. Methods BMSCs of rabbits were extracted and proliferation capacity was detected. After BMSCs were cultured in adipogenesis medium supplemented with or without irisin, qPCR was used to evaluate the expression levels of PPARγ and C/EBPα, while oil red O staining was used to investigate fat accumulation. The signaling protein level of Wnt10a was detected by Western blot. Results Proliferation capacity of BMSCs was hardly affected by irisin. Irisin could significantly downregulate mRNA expressions of PPARγ [(0.20±0.06) vs (1.00±0.10) 7 days after adipogenesis induction and(0.61±0.06) vs (1.00±0.15) 14 days after adipogenesis induction]and C/EBPα [(0.40±0.06) vs (1.00±0.12) 7 days after adipogenesis induction and (0.70±0.09) vs (1.00±0.18) 14 days after adipogenesis induction], while attenuating fat accumulation [(0.45±0.05) vs (0.95±0.10),P<0.05]. Moreover, Wnt10a protein levels were increased by irisin intervention[(5.01±0.78) vs (1.00±0.25),P<0.05]. Conclusions Irisin can inhibit adipocyte differentiation of BMSCs from rabbits, and this effect is related to activation of Wnt signaling pathway.
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2020, Vol. 31 
