1. Department of Stomatology, Beijing Railway Construction Hospital, Beijing 100855, China; 2. Department of Stomatology, the First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; 3. Medical Department, Guangzhou Geriatric Hospital, Guangzhou 510000, China; 4. Department of Stomatology, the Third Medical Center of Chinese PLA General Hospital, Beijing 100039, China
Abstract：Objective To investigate the effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells (BMSCs) in the inflammatory microenvironment.Methods BMSCs were isolated, cultured and divided into 3 groups: Vehicle, LPS and Irisin (LPS plus irisin intervention). CCK-8 was used to access cell proliferation. After cultured in osteogenic differentiation medium, ALP activity was evaluated, mRNA levels of Runx2, Osx, ALP and OCN and protein levels of ALP and OCN were also investigated. Moreover, alizarin red was used to determine mineralization level.Results Compared with the Vehicle group, LPS significantly promoted BMSCs proliferation (P＜0.05); LPS downregulated Runx2, Osx, ALP and OCN mRNA expression and reduced ALP and OCN protein levels, while irisin intervention could obviously upregulate Runx2, Osx and ALP mRNA expression, enhanced ALP and OCN protein levels, and raised mineralization level (P＜0.05). Conclusions Irisin can promote osteoblast differentiation of BMSCs in the inflammatory microenvironment.
Redlich K, Smolen J S. Inflammatory bone loss: pathogenesis and therapeutic intervention[J]. Nat Rev Drug Discov, 2012,11(3):234-250.
[2]
Souza P P, Lerner U H. The role of cytokines in inflammatory bone loss[J]. Immunol Invest, 2013,42(7):555-622.
[3]
Arango D G, Descoteaux A. Macrophage cytokines: involvement in immunity and infectious diseases[J]. Front Immunol, 2014,5:491.
[4]
Lu J, Xiang G, Liu M, et al. Irisin protects against endothelial injury and ameliorates atherosclerosis in apolipoprotein E-Null diabetic mice[J]. Atherosclerosis, 2015,243(2):438-448.
Shao L, Meng D, Yang F, et al. Irisin-mediated protective effect on LPS-induced acute lung injury via suppressing inflammation and apoptosis of alveolar epithelial cells[J]. Biochem Biophys Res Commun, 2017,487(2):194-200.
[8]
Zhu J, Tang H, Zhang Z, et al. Kaempferol slows intervertebral disc degeneration by modifying LPS-induced osteogenesis/adipogenesis imbalance and inflammation response in BMSCs[J]. Int Immunopharmacol, 2017,43: 236-242.
Yu X, Hu Y, Freire M, et al. Role of toll-like receptor 2 in inflammation and alveolar bone loss in experimental peri-implantitis versus periodontitis[J]. J Periodontal Res, 2018,53(1):98-106.
[14]
Pogozhykh O, Pogozhykh D, Neehus A L, et al. Molecular and cellular characteristics of human and non-human primate multipotent stromal cells from the amnion and bone marrow during long term culture[J]. Stem Cell Res Ther, 2015,6(1):150.
[15]
Shi Q, Qian Z, Liu D, et al. GMSC-derived exosomes combined with a chitosan/silk hydrogel sponge accelerates wound healing in a diabetic rat skin defect model[J]. Front Physiol, 2017,8:904-906.
[16]
Einhorn T A, Gerstenfeld L C. Fracture healing: mechanisms and interventions[J]. Nat Rev Rheumatol, 2015,11(1): 45-54.
[17]
Polyzos S A, Kountouras J, Anastasilakis A D, et al. Irisin in patients with nonalcoholic fatty liver disease[J]. Metabolism, 2014,63(2):207-217.
[18]
Chen X, Sun K, Zhao S, et al. Irisin promotes osteogenic differentiation of bone marrow mesenchymal stem cells by activating autophagy via the Wnt//β-catenin signal pathway[J]. Cytokine, 2020,136:155292.
[19]
Zeng M, Sang W, Chen S, et al. 4-PBA inhibits LPS-induced inflammation through regulating ER stress and autophagy in acute lung injury models[J]. Toxicol Lett, 2017,271:26-37.
[20]
Qu L, Chen C, He W, et al. Glycyrrhizic acid ameliorates LPS-induced acute lung injury by regulating autophagy through the PI3K/AKT/mTOR pathway[J]. Am J Transl Res, 2019,11(4):2042-2055.
[21]
Larabi A, Barnich N, Nguyen H. New insights into the interplay between autophagy, gut microbiota and inflammatory responses in IBD[J]. Autophagy, 2020,16(1):38-51.
[22]
Cao Y, Chen J, Ren G, et al. Punicalagin prevents inflammation in LPS-induced RAW264.7 macrophages by inhibiting FoxO3a/autophagy signaling pathway[J]. Nutrients, 2019,11(11):2794.
2023, Vol. 34 
