脂联素对钙化大鼠血管组织的影响

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Abstract Objective To observe the changes of adiponectin and its receptors in vascular calcification, and the role of adiponectin on calcified tissues. Methods Male SD rats were induced vascular calcification by vitamin D3 and nicotine, with or without adiponectin. Calcium and alkaline phosphatase (ALP) were assayed. The levels of APN in plasma and vascules were measured by ELISA. The mRNA levels of APN and adipo R1 were detected by using real-time PCR, the level of adipo R1 protein was detected by Western blotting. Results Compared with control group, the APN concentrations in plasma and calcified blood vessels decreased by 47.1% and 57.1%, respectively. The mRNA levels of APN and adipo R1 decreased by 65.0% and 72.0%.The adipo R1 protein decreased by 77.2%(P<0.01). 6.55 fold increase calcium and 83.4% increase in ALP activity were observed in calcified blood vessels than that in control group, while APN treatment down-regulated the calcium and ALP activity by 68.5% and 34.8% (P<0.01). Conclusions In calcified blood vessels, the APN and adipo R1 system are down-regulated, and APN decreases the calcium and ALP activity, suggesting that APN may have cardiovascular protection by antagonizing calcification.

Key words： vascular calcification adiponectin adipo R1

Received: 10 March 2015

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	YAN Liang
	WANG Kun

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YAN Liang,WANG Kun. Influence of adiponectin on vascular calcification in rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(8): 808-811.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I8/808

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