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| Method validation of multipotential differentiation of human induced pluripotent stem cells in vitro |
| FENG Dingli1,2, ZHUO Lidan1, LU Di3, LI Hong4, GUO Hongyan1 |
1.Dental Department,The Third Medical Center of PLA General Hospital, Beijing 100039, China;
2. Stomatological Hospital of Stomatology, Shanxi Medical University, Taiyuan 030001, China;
3. Plastic Surgery Department of PLA General Hospital, Beijing 100853, China;
4. Institute of Military Medicine, Academy of Military Sciences, Beijing 100850, China |
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Abstract Objective To study the multi-directional differentiation ability of induced pluripotent stem cells (iPSC). Methods iPSCs were induced into Mesenchymal stem cells (MSCs)-like cells by the formation of Embryonic body EBs. The morphology of cells was observed under inverted microscope. Flow cytometry was performed. Expression of surface markers of iPSC-derived MSCs (iPS-MSCs), and further induction of iPSC-MSCs into osteoblasts, chondrocytes; or direct EB inoculation, which induces them into nerve cells. The multi-directional differentiation ability of iPSC was verified by the above method. Results After induction, iPSC-MSC gradually grew into a long spindle shape; CD29 and CD105 were positive in iPSC-MSCs, while CD34 and CD45 were negative; alkaline phosphatase and toluidine staining showed iPSC- MSCs have the ability to form osteoblasts and cartilage; iPSCs can also be induced into neurons directly from the EB stage. Conclusions Through the above methods, iPSC can be successfully induced into osteoblasts, cartilage and neuroblasts, which provides a technical basis for further research and application of iPSC.
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Received: 05 December 2018
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2019, Vol. 30 
