胆囊癌差异蛋白质组学分析

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Abstract Objective To identify the differential proteins in gallbladder cancer group and normal gallbladder tissue control group, and find and identify the tumor markers related to gallbladder cancer and potential therapeutic targets by using classical proteomic techniques, two-dimensional gel electrophoresis, mass spectrometry identification and bioinformatics analysis. Methods The samples were divided into two groups: gallbladder cancer group (59 cases) and normal control group (40 cases). The total protein samples were collected by washing, lysis and centrifugation of the tissue samples, and then packaged and frozen. After the first direction isofocal focusing and the second SDS-PAGE electrophoresis, the gel was dyed and colored with Coomnassie blue, and the gel image was obtained by Gs-800 optical densitometer. After analysis by PD Quest gel image analysis software, different protein spots were found. Secondary mass spectrometry of differential protein fragments was obtained using Peptide Mass Fingerprinting (PMF) and Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry techniques. Biotools software was used to collect mass- charge ratio information of peptide fragments. The Mascot search engine was used to search the database, identify the amino acid sequences of differential proteins, and obtain the corresponding specific protein information. Results A total of 66 differentially expressed proteins were found, Among which, 21 proteins were up-regulated and 45 proteins were down-regulated. Of 21 differential proteins with scores higher than 56, 10 were up-regulated and 11 were down-regulated in gallbladder cancer. It mainly includes annexinsANX, moesin, heat shock protein (HSP), vimentin, Retinol dehydrogenase 1, galectin, and so on. Conclusions The above-mentioned proteins may play an important role in the occurrence and development mechanism of tumors, and are potential targets for diagnosis and treatment, which deserves further research in the future.

Key words： gallbladder cancer tissue proteins two dimensional gel electrophoresis mass spectrometry

Received: 10 October 2023

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	HUANG Hailin
	YANG Zhigang

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HUANG Hailin,YANG Zhigang. Differential proteomic analysis of gallbladder cancer[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2024, 35(4): 318-324.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2024/V35/I4/318

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