Changes in contents of leukocyte mitochondrial DNA and oxidative damage during acclimatization to highland
BO Hai1,2,GUO Fengchun3,DUAN Fuqiang4,PENG Peng1,QIN Yongsheng1
1.Department of Military Training Medicine, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300309, China;
2.Tianjin Key Laboratory of Cardiovascular Remodeling and Target Organ Injury, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300309, China;
3.Department of Logistics Research, Logistics University of Chinese People’s Armed Police Forces, Tianjin 300309, China;
4.Department of Health, Tibet Autonomous Regional Corps Hospital of Chinese People’s Armed Police Forces, Lhasa 850000, China
Abstract：Objective To study the changes in mitochondrial bioenergetics, mitochondrial DNA (mtDNA) and oxidative damage in leukocytes during acclimatization to highland hypoxia. Methods Twenty-seven lowlanders quickly entered plateau. Leukocyte mitochondrial membrane potential, 8-oxodG in mtDNA, mtDNA copies and PGC-1α protein expression were measured in plain and plateau at 3 d,7 d,90 d after entry. Results As compared with plain group, membrane potential was significantly decreased (P<0.05); 8-oxodG amounts, mtDNA copies and PGC-1α expression were markedly increased at 3 d and 7 d (P<0.05). And at 90 d, mtDNA copies and PGC-1α expression were significantly decreased (P<0.05). As compared with 7 d group, membrane potential was significantly increased (P<0.05); 8-oxodG amounts, mtDNA copies and PGC-1α expression were markedly decreased at 90 d (P<0.05). Conclusions In the early phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on increased mitochondrial content. In the later phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on elevated level of single mitochondrial health and energy metabolism.
薄海,果凤春,段富强,彭朋,秦永生. 高原习服过程淋巴细胞线粒体DNA含量及氧化损伤变化规律[J]. 武警医学, 2014, 25(8): 761-764.
BO Hai,GUO Fengchun,DUAN Fuqiang,PENG Peng,QIN Yongsheng. Changes in contents of leukocyte mitochondrial DNA and oxidative damage during acclimatization to highland. Med. J. Chin. Peop. Armed Poli. Forc., 2014, 25(8): 761-764.
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2014, Vol. 25 
