高强度间歇运动训练对急进高原淋巴细胞线粒体自噬的影响

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摘要目的观察平原短期高强度间歇运动训练(high-intensity interval training, HIT)对青年男性急进高原淋巴细胞线粒体自噬的影响。方法 30名世居平原青年男性随机分为无运动训练组(NT组,n=15)和HIT组(n=15)。HIT组受试者进行HIT训练,以90%~95%心率贮备强度快跑3 min,间歇以45%~50%心率贮备强度慢跑3 min,重复6个循环,1次/d,共2周。NT组除日常活动外不进行任何形式运动训练。训练后所有受试者同批次急进高原,在移居高原24 h检测淋巴细胞线粒体复合体活性,以及PINK1、淋巴细胞线粒体自噬蛋白Bnip3和Beclin-1蛋白表达。结果与NT组比较,HIT组Bnip3(100±18 vs 136±32)和Beclin-1(100±19 vs 119±26)表达明显升高(P均＜0.05),线粒体复合体Ⅰ、Ⅱ、Ⅳ和ATP合成酶活性也明显升高(P＜0.05),PINK1蛋白表达明显降低(P＜0.01)。结论平原阶段短期HIT可通过上调线粒体自噬阈,从而减少高原低氧环境下淋巴细胞线粒体损伤,提高线粒体能量代谢水平。

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	刘静
	张红英
	段富强
	彭朋
	秦永生
	薄海

关键词 ：高强度间歇运动训练, 高原低氧, 淋巴细胞, 线粒体自噬, 能量代谢

Abstract：Objective To explore the effect of short-term high-intensity interval training (HIT) in plain on mitochondrial autophagy in leukocyte after rush entry into plateau. Methods Thirty male lowlanders were randomly divided into two groups: untrained group (NT, n=15) and HIT group (n=15). Participants in the HIT group run at a high training intensity, 90%-95% heart rate reserve (HRR) for 3 min, with active pauses of 3 min of walking at 45%-50% of HRR. Participants in the HIT group underwent six sessions of HIT for 36 min/d, 7 days per week for 2 weeks. After training, participants in both HIT and NT groups traveled into plateau in the same batch. Activities of mitochondrial complex, PINK1, Bnip3 and Beclin-1 protein expression in leukocyte were measured in plateau at 24 h after entry. Results As compared with NT group, in HIT group, mitochondrial autophagy proteins Bnip3 (100±18 vs 136±32) and Beclin-1(100±19 vs 119±26) expression were significantly elevated(P<0.05). Furthermore, mitochondrial complex Ⅰ, Ⅱ, Ⅳ and ATP synthase activities were significantly elevated (P<0.05), whereas PINK1 protein expression was significantly decreased (P<0.01). Conclusions Short-term HIT in plain can blunt high altitude hypoxia induced mitochondrial impariment and elevated mitochondrial energy metabolism through upregulation of mitochondrial autophagy.

Key words： high-intensity interval training high altitude hypoxia leukocyte mitochondrial autophagy energy metabolism

收稿日期: 2014-09-22

ZTFLH:

R594.3

基金资助:国家自然科学基金青年基金(31200894);天津市应用基础及前沿技术研究计划青年基金(12JCQNJC07900)

通讯作者: 薄海,E-mail:bohaixd@126.com E-mail: jingjingliuliu@126.com

作者简介: 刘静,博士,讲师

引用本文:

刘静,张红英,段富强,彭朋,秦永生,薄海. 高强度间歇运动训练对急进高原淋巴细胞线粒体自噬的影响[J]. 武警医学, 2014, 25(12): 1228-1231. LIU Jing, ZHANG Hongying, DUAN Fuqiang, PENG Peng, QIN Yongsheng, and BO Hai. Effect of high-intensity interval training on mitochondrial autophagy in leukocyte after rush entry into plateau. Med. J. Chin. Peop. Armed Poli. Forc., 2014, 25(12): 1228-1231.

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http://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 http://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2014/V25/I12/1228

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