Abstract：Objective To study transplantation of bone mesenchymal stem cells (BMSCs) derived neuron-like cells for repairing the rat spinal cord injury. Methods BMSCs were isolated from the femur of 4-week-old Sprague-Dawley rats, after culture and proliferation, then differentiated into neuron-like cells induced by retinoic acid (RA). After spinal cord injury (SCI) model preparation, forty-eight rats were randomly assigned to the following three groups (n=18): the neuron-like cells group, the BMSCs group and the control group. The rats’ motor functional recovery was evaluated by BBB score and Rivlin platform test at 2 w, 4 w, 6 w after operation. Results The neuron-like cells derived from BMSCs had some neurocyte characteristics. Transplantation of neuron-like cells(16.56±2.42) showed higher score than BMSCs(13.70±2.66). The rat functional recovery in the neuron-like cells group and the BMSCs group was better than in the control group. There was more nerve fibers with normal structure could be observed in the neuron-like cells group. Conclusions Transplantation of neuron-like cells derived from BMSCs for treating rat spinal cord injury is a promising treatment.
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2016, Vol. 27 
