Effects of quercetin on proliferation of hypoxia induced hepatoma carcinoma cell HepG2 and expressions of HIF-1α 、VEGF
WEI Yan1, LU Yanling1, WANG Rongrong1, FENG Shengjin1, LIANG Gang2
1.Pharmacy Department of Guangxi Rogional Crops Hospital of Chinese People’s Armed Police Force,Nanning 530003 China 2.Department of Pharmacology,Pharmacy College of Guangxi Medical University,Nanning 530021, China
Abstract：Objective To explore the effect of quercetin on the proliferation and expression of HIF-1α and VEGF , and the mechanisms in hypoxia hepatoma carcinoma cell HepG2.Methods The cells were divided into normal control group , hypoxia group and hypoxia combined with quercetin group. A hypoxia model was established using cobalt chloride (CoCl2).The proliferation of cells was determined by MTT assay.The mRNA expression and protein expression of HIF-1α and VEGF were determined by real-time quantification PCR and Western blot.Results Compared with the normal control group, the proliferation of the hypoxia group was decreased at 24, 48 and 72 h(P<0.05),while the proliferation in hypoxia combined with quercetin group was decreased at 24,48 and 72 h(P<0.05)compared with the hypoxia group.The optical density was 0.640±0.016、1.287±0.025 and 1.738±0.027 respectively in normal control group, 0.551±0.021、0.851±0.028 and 1.268±0.018 in hypoxia group, and 0.435±0.023、0.717±0.013 and 0.984±0.037 respectively in hypoxia combined with quercetin group.The mRNA and protein expressions of HIF-1α and VEGF in hypoxia group were increased(P<0.05)compared with normal control group. Quercetin down-regulated the mRNA and protein expressions of VEGF(P<0.05)in hypoxia cells,but had no effect on the mRNA and protein expression of HIF-1α (P>0.05).The mRNA expressions of HIF-1α and VEGF were both 1.000±0.000 and the protein expressions were 0.616±0.016 and 0.831±0.034 in normal control group. The protein expressions were 1.385±0.047 and 2.022±0.076 in the hypoxia group, but were 1.406±0.029 and1.137±0.054 in the hypoxia combined with quercetin group.Results Quercetin can inhibit the proliferation of hepatoma carcinoma cell HepG2 under hypoxia conditions,which may be related to down-regulated expressions of VEGF.
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2018, Vol. 29 
