Effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress
LEI Jing1, ZHANG Xinyu1, CHEN Lijun1, WANG Daning2, MENG Bin3, WEN Xiaochang1, WANG Jiangping1
1. Department of Human Functional Physiology, 2. Department of Military Training Medicine, 3. Department of Military Preventive Medicine, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, China
Abstract：Objective To explore the effect of resistant dextrin on gut microbiota in rats with chronic unpredicted mild stress.Methods Twenty-four male SD rats were randomly divided into a control group, a model group and a resistant dextrin group. A depression-like rat model was established using chronic unpredictable mild stress (CUMS). After 3 weeks of stress modelling, sucrose preference test and open- field test were used to evaluate the depression-like behavior of rats. Stress sensitive rats were screened in the model group, and the same number of rats in the other two groups were randomly selected to detect the composition and structural changes of gut microbiota by 16S rRNA sequencing.Results Compared with the control group, relative abundance of Firmlcutes [(0.92251±0.02838) vs. (0.82572±0.00761)], Akkermansia[(0.01327±0.00076) vs. (0.00000±0.00000)]and Lactococcus [(0.00029±0.00010) vs. (0.00000±0.00000)] in the model group was significantly reduced (P<0.05); relative abundance level of Bacteroidetes (0.04671±0.00263) vs. (0.12341±0.03576), Faecalibacterium[(0.00011±0.00004)vs.(0.00057±0.00005)]and Ruminococcaceae_UCG_014 [(0.01003±0.00384)vs.(0.10976±0.04364)] was significantly increased (P<0.05). Compared with the model group, resistant dextrin could significantly callback the microflora with changed abundance (P<0.05).Conclusions Resistant dextrin can regulate the gut microbiota of CUMS rats and restore intestinal dynamic balance by improving the relative abundance of probiotics and reducing the relative abundance of pathogenic bacteria, which provides experimental basis for the treatment of depression.
Jiang H, Ling Z, Zhang Y, et al. Altered fecal microbiota composition in patients with major depressive disorder[J]. Brain Behav Immun, 2015, 48: 186-194.
[2]
Osadchiy V, Martin C R, Mayer E A. The Gut-Brain Axis and the Microbiome: mechanisms and clinical implications[J]. Clin Gastroenterol Hepatol, 2019,17(2):322-332.
[3]
Zheng P, Zeng B, Zhou C, et al. Gut microbiome remodeling induces depressive-like behaviors through a pathway mediated by the host's metabolism[J]. Mol Psychiatry,2016, 21(6): 786-796.
[4]
Kelly J R, Borre Y, O' Brien C, et al. Transferring the blues: depression-associated gut microbiota induces neurobehavioural changes in the rat[J]. J Psychiatr Res,2016, 82: 109-118.
[5]
Qiao Y, Zhao J, Li C, et al. Effect of combined chronic predictable and unpredictable stress on depression-like symptoms in mice[J]. Ann Transl Med, 2020, 8(15): 942.
[6]
Zhu S, Jiang Y, Xu K, et al. The progress of gut microbiome research related to brain disorders[J]. J Neuroinflammation, 2020,17(1):25.
[7]
Yang Z, Li J, Gui X, et al. Updated review of research on the gut microbiota and their relation to depression in animals and human beings[J]. Mol Psychiatry, 2020, 25(11):2759-2772.
Hobden M R, Guérin-Deremaux L, Rowland I, et al. Potential anti-obesogenic properties of non-digestible carbohydrates: specific focus on resistant dextrin[J]. Proc Nutr Soc, 2015, 74(3): 258-267.
[10]
Parnell J A, Reimer R A. Prebiotic fiber modulation of the gut microbiota improves risk factors for obesity and the metabolic syndrome[J]. Gut Microbes, 2012, 3(1): 29-34.
Czéh B, Fuchs E, Wiborg O, et al. Animal models of major depression and their clinical implications[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2016,64:293-310.
[15]
Antoniuk S, Bijata M, Ponimaskin E, et al. Chronic unpredictable mild stress for modeling depression in rodents: meta-analysis of model reliability[J]. Neurosci Biobehav Rev, 2019, 99:101-116.
Gourley S L, Swanson A M, Koleske A J. Corticosteroid-induced neural remodeling predicts behavioral vulnerability and resilience[J]. J Neurosci, 2013, 33(7):3107-3112.
[20]
Aizawa E, Tsuji H, Asahara T, et al. Possible association of Bifidobacterium and Lactobacillus in the gut microbiota of patients with major depressive disorder[J].J Affect Disord,2016, 202:254-257.
Yu M, Jia H, Zhou C, et al. Variations in gut microbiota and fecal metabolic phenotype associated with depression by 16S rRNA gene sequencing and LC/MS-based metabolomics[J]. J Pharm Biomed Anal, 2017, 138: 231-239.
Swidsinski A, Dörffel Y, Loening-Baucke V, et al. Acute appendicitis is characterised by local invasion with Fusobacterium nucleatum/necrophorum[J].Gut, 2011, 60(1):34-40.
[25]
Belzer C, de Vos W M. Microbes inside--from diversity to function: the case of Akkermansia[J].ISME J, 2012, 6(8):1449-1458.
[26]
Wu M, Tian T, Mao Q, et al. Associations between disordered gut microbiota and changes of neurotransmitters and short-chain fatty acids in depressed mice[J]. Transl Psychiatry, 2020, 10(1):350.
[27]
Wu S J, Chang C Y, Lai Y T, et al. Increasing γ-aminobutyric acid content in vegetable soybeans via high-pressure processing and efficacy of their antidepressant-like activity in mice[J]. Foods, 2020, 9(11): 1673.
[28]
Valles-Colomer M, Falony G, Darzi Y, et al. The neuroactive potential of the human gut microbiota in quality of life and depression[J]. Nat Microbiol, 2019, 4(4):623-632.
[29]
Huang T T, Lai J B, Du Y L, et al. Current understanding of gut microbiota in mood disorders: an update of human studies[J]. Front Genet, 2019, 10:98.
[30]
Qu Y, Su C, Zhao Q, et al. Gut microbiota-mediated elevated production of secondary bile acids in chronic unpredictable mild stress[J]. Front Pharmacol, 2022, 13:837543.
2023, Vol. 34 
