高压氧干预对大鼠创伤性脑损伤后脑组织NF-κB (p50)表达的影响

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Abstract Objective To observe the effect of hyperbaric oxygenation treatment(HBOT) on expression of NF-κB(p50) in the rat brain tissue after traumatic brain injury(TBI) and discuss its mechanism. Methods Thirty-five Sprague-Dawley rats were divided into sham operation control group(5 rats), TBI group(15 rats) and HBOT group(15 rats). Based on the Feeney’s model of modified Allen’s method, experimental animals were treated with HBOT. The animals were sacrificed at the 8th hour, and on days 1,3,5,8,respectively. The expression of protein NF-κB was determined by Westernblotting. Results The expression of NF-κB was significantly higher in traumatic group than that in control group at the time of 8 hour and 1,3,5and 8 days after injury (P＜0.05).The level of NF-κB reached peak on the 3th day, and maintained a high status from 5 to 8 days. Compared with traumatic group, the NF-κB level was significantly lower in the HBOT group (P＜0.05). Conclusion HBOT can weaken NF-κB expression in traumatic brain tissue and inhibit effectively inflammatory response, which provides theoretical basis for treating severe traumatic brain injury.

Key words： severe brain injury hyperbaric oxygenation rats NF-κB

Received: 26 June 2014

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R651.11

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	Articles by authors
	YANG Bolin
	WANG Huan
	PENG Jun
	XU Qiming
	and YU Chao

Cite this article:

YANG Bolin,WANG Huan,PENG Jun等. Effect of hyperbaric oxygenation treatment on expression of NF-κB p50 in the rat brain tissue after traumatic brain injury[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2014, 25(11): 1131-1134.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2014/V25/I11/1131

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