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| Effect of naringenin on energy metabolism in rats with myocardial ischemia/reperfusion injury |
| QIAN Xiufang1, GUO Zhe1, HU Changju1, LIU Dan1, and ZHANG Guobin2 |
1.Department of Pharmacy, the Second Hospital of Beijing Municipal Corps, Chinese People’s Armed Police Force, Beijing 100027, China;
2. Pharmacy College, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China |
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Abstract Objective To investigate the effect of naringenin on energy metabolism in rats with myocardial ischemia/reperfusion injury.Methods SD rats were divided randomly into sham group, model group, naringenin low dose group (25 mg/kg), naringenin medium dose group (50 mg/kg) and naringenin high dose group (100 mg/kg) (n=10). Naringenin was intraperitoneally injected once a day for 7 days before the modeling operation. A myocardial ischemia/reperfusion injury model was established by coronary artery ligation (30 min) and subsequent reperfusion (2 hours) in rats. After the modeling operation, the CK and LDH activities in sera were examined by chromatometry, Na＋/K＋-ATPase and Ca2+/Mg2+-ATPase activities in brain tissues were detected by phosphorus method and the myocardial infarction size (MIS) was determined by staining method.Results A high dose of naringenin significantly reduced MIS to 32.91% compared to 39.78% of model group (P<0.05). The CK activities in naringenin groups were 658.03, 650.12 and 621.89 U/L, respectively, which were significantly lower than 809.45 U/L of model group (P<0.05 or P<0.01). Naringenin significantly reduced LDH activities to 1543.08, 1506.27 and 1326.97 U/L, respectively, compared to 2034.56 U/L of model group (P<0.05 or P<0.01). Naringenin evidently increased Na＋/K＋-ATPase activities to 6.82, 6.83 and 6.94 mmol Pi/(g·h), respectively, compared to 5.54 mmol Pi/(g·h) of model group (P<0.05). Naringenin (100 and 50 mg/kg) significantly increased Ca2+/Mg2+-ATPase activities to 8.42 and 8.97 mmol Pi/(g·h), respectively, compared to 7.06 mmol Pi/(g·h) of model group (P<0.05).Conclusions The protective effects of naringenin pretreatment in myocardial ischemia/reperfusion injury seems to be associated with the improvement of energy metabolism.
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Received: 09 January 2017
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2017, Vol. 28 
