富氢盐水抑制大鼠压疮深部骨骼肌NLRP3炎性小体活化

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Abstract Objective To explore the effect of hydrogen-rich saline on the oxidative stress and inflammation in skeletal muscle of pressure ulcer model rats, and to investigate the biological effect of NLRP3 inflammasome.Methods Thirty male Sprague Dawley rats were randomly divided into three groups: control group (C), pressure ulcer + saline group (PU+S), and pressure ulcer + hydrogen-rich saline group (PU +HS). The PU+S and PU +HS rats were loaded with 22.47kPa pressure and kept 2 hours for simulation of ischemia, and simulate reperfusion 30 minutes later. Every rat was performed this treatment 5 cycle everyday, for 3 days. After pressure ulcer model established, PU+HS rats subjected to intraperitoneal injection of hydrogen-rich saline (10 ml/kg) for 5 days. Then MDA level, IL-1β content, Caspase-1 activity, NLRP3 and ASC protein expression were measured. Results As compared with PU+S group, in PU+HS group, MDA level, IL-1β content and Caspase-1 activity were significantly decreased (14.92±3.11 vs 27.40±4.42,1.13±0.25 vs 1.42±0.35 and 0.69±0.18 vs 1.05±0.19, respectively, P<0.05~0.01). Furthermore, NLRP3 and ASC protein expression were significantly suppressed (122.29±19.96 vs 178.49±18.62 and 105.33±16.67 vs 129.49±18.77, respectively, P<0.01).Conclusion Hydrogen-rich saline could prevent NLRP3 inflammasome activation through suppressing oxidative stress, which in turn inhibit pressure ulcer induced inflammation in skeletal muscle.

Key words： hydrogen-rich saline pressure ulcer skeletal muscle NLRP3 inflammasome oxidative stress

Received: 21 April 2018

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R758.19

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	CHEN Xiaowei
	ZHANG Hongying
	LIU Jing

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CHEN Xiaowei,ZHANG Hongying,LIU Jing. Hydrogen-rich saline inhibits NLRP3 inflammasome activation in skeletal muscle of pressure ulcer model rats[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2018, 29(8): 747-749.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2018/V29/I8/747

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