Method validation of multipotential differentiation of human induced pluripotent stem cells in vitro
FENG Dingli1,2, ZHUO Lidan1, LU Di3, LI Hong4, GUO Hongyan1
1.Dental Department,The Third Medical Center of PLA General Hospital, Beijing 100039, China; 2. Stomatological Hospital of Stomatology, Shanxi Medical University, Taiyuan 030001, China; 3. Plastic Surgery Department of PLA General Hospital, Beijing 100853, China; 4. Institute of Military Medicine, Academy of Military Sciences, Beijing 100850, China
Abstract：Objective To study the multi-directional differentiation ability of induced pluripotent stem cells (iPSC). Methods iPSCs were induced into Mesenchymal stem cells (MSCs)-like cells by the formation of Embryonic body EBs. The morphology of cells was observed under inverted microscope. Flow cytometry was performed. Expression of surface markers of iPSC-derived MSCs (iPS-MSCs), and further induction of iPSC-MSCs into osteoblasts, chondrocytes; or direct EB inoculation, which induces them into nerve cells. The multi-directional differentiation ability of iPSC was verified by the above method. Results After induction, iPSC-MSC gradually grew into a long spindle shape; CD29 and CD105 were positive in iPSC-MSCs, while CD34 and CD45 were negative; alkaline phosphatase and toluidine staining showed iPSC- MSCs have the ability to form osteoblasts and cartilage; iPSCs can also be induced into neurons directly from the EB stage. Conclusions Through the above methods, iPSC can be successfully induced into osteoblasts, cartilage and neuroblasts, which provides a technical basis for further research and application of iPSC.
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2019, Vol. 30 
