Tanshinone ⅡA inhibits brain injury caused by lung blast injury Protective effect of Tanshinone ⅡA on brain injury induced by lung blast injury in mice
SHI Lin, TONG Changci, CONG Peifang, LIU Yunen
Liaoning key Laboratory of severe Trauma and Organ Protection, Northern Theater General Hospital of PLA, Shenyang 110016, China
Abstract：Objective To investigate the protective effect of Tanshinone ⅡA on brain injury induced by lung blast injury in mice.Methods Thirty C57BL/6 mice were randomly divided into control group (Sham), lung blast injury group (Blast) and TanⅡA intervention group. Samples were collected 48 hours after blast injury, and the pathological changes of brain tissue were observed by HE and ROS staining. The expressions of brain injury markers Tau and S100β, inflammation-related factors IL-1β, TNF-α, and IL-10, oxidative stress related factors SOD-1, IRE-αand MDA5, as well as PI3K, p-PI3K, Akt, p-Akt and NF-κB were detected by Western blot.Results Compared with sham group, S100 β increased to (31.5±4.19), other related promoters increased, while NF-κB increased to (12.25±1.92), PI3K and AKT phosphorylation increased to (4.36±0.41) and (27.00±0.09), respectively. Compared with blast group, S100β decreased to (0.21±0.09) in Tan IIA group, TanⅡA restored the changes of related factors induced by shock wave, and NF-κB decreased to (0.32±0.02), PI3K and AKT phosphorylation decreased to (0.47±0.01) and (0.18±0.04) respectively, the differences were statistically significant (P<0.05).Conclusions TanⅡA has a protective effect on brain injury caused by lung blast injury, and its mechanism may be achieved by inhibiting PI3K/Akt/NF-κB signal pathway.
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2021, Vol. 32 
