Effect of flagellum protein derivative CZLC331 on the depressive behavior of mice with ulcerative colitis
WANG Huan1, XU Yang1, QU Yawei1, ZHANG Chenggang2, LIU Haifeng1
1.Department of Gastroenterology, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China; 2.Institute of Radiation Medicine, Academy of Military Medical Sciences; State Key Laboratory of Proteomics; Research Center for Military Cognition and Mental Hygiene of Chinese PLA, Beijing 100850, China
Abstract：Objective To study the effect of flagellum protein derivative CZLC331 on the depressive behavior of mice with ulcerative colitis. Methods UC mice model was established by TNBS enema. Eighteen SPF male Balb/c mice were randomly and equally divided into the control group, UC model group, and CZLC331 treatment group, six mice in each group. Mice depression index was observed by forced swimming test, tail suspension experiment and sugar water consumption experiment. Results For the forced swimming test and tail suspension test, the immobility time in treated mice [(64.7±10.4)s,(62.5±13.9)s]was significantly lower than in the model group[(86.7±16.0)s,(111.3±41.1)s], and significantly higher than in the control group[(21.0±5.3)s,(16.0±8.3)s]. In sugar water consumption experiment, the percentage of sucrose preference in treated mice (66.5±7.3)% was significantly higher than in model group(34.5±4.6)%, and significantly lower than in the control group(77.2±7.7)%. Conclusions CZLC331 can effectively improve the level of depression in mice with ulcerative colitis.
王寰, 徐阳, 屈亚威, 张成岗, 刘海峰. 细菌鞭毛蛋白衍生物CZLC331对溃疡性结肠炎小鼠抑郁行为的影响[J]. 武警医学, 2015, 26(4): 329-331.
WANG Huan, XU Yang, QU Yawei, ZHANG Chenggang, LIU Haifeng. Effect of flagellum protein derivative CZLC331 on the depressive behavior of mice with ulcerative colitis. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(4): 329-331.
Yamamoto-Furusho J K, Uscanga L F, Vargas -Alarcón G, et al. Clinical and genetic heterogeneity in Mexican patients with ulcerative colitis[J]. Hum Immunol,2003,64(1):119-123.
[3]
Campieri M , Gionchetti P. Bacteria as the cause of ulcerative colitis [J]. Gut, 2001, 48(1): 132-135.
Burdelya LG, Krivokrysenko V I, Tallant T C, et al.An agonist of toll-like receptor 5 has radioprotective activity in mouse and primate models[J].Science,2008,320(5873):226-230.
[6]
Huser W,Janke K H,Klump B,et al.Anxiety and depression in pa- tients with inflammatory bowel disease: comparisons with chronic liver disease patients and the general population [J].Inflamm Bowel Dis,2011,17(2):621-632.
[7]
Cheng W F,Shi R H.Investigation on psychological characteristics in ulcerative colitis[J].Chin J Gastroenterol Hepatol,2007,16(5):437-439.
[8]
Hossain M S, Gewirtz A T, Roback J D, et al.Flagellin A TLR5 agonist, facilitates production of foxp3+CD4+CD25+regulatory T cells to maintain balanced immune reconstitution in allogeneic BMT without GvHD[J].Biol Blood Marrow Tr, 2009,15(2):12-15.
[9]
Lyudmila G, Burdelya , Tallant T C, et al. An agonist of Toll-like receptor 5 has radioprotective activity in mouse and primate models [J] . Science, 2008,320(5873):226-230.
[10]
Vijay-Kumar M , Aitken J D, Sanders C J, et al.Flagellin treatment protects against chemicals, bacteria, viruses, and radiation[J].lmmunol, 2008,180(12):8280- 8285.
[11]
Jackson L N, Zhou Y N, Qiu S M, et al. Alternative medicine products as a novel treatment strategy for inflammatory bowel disease[J]. Am J Chin Med, 2008,36(5):953-965.
2015, Vol. 26 
