Role of microRNA-22 regulating chondrocyte autophagy in pathogenesis of osteoarthritis
YAN Shiju1, DONG Wenjing2, LI Zhirui1, ZHAO Yanpeng3, HAN Tao1, WEI Junqiang1, WANG Junliang1, LIN Feng1
1. Department of Orthopedics, 2. Department of Gerontology, Hainan Hospital of Chinese PLA General Hospital, Sanya 572013, China; 3. Department of Orthopedics, the Fourth Medical Center of Chinese PLA General Hospital, Beijing 100048, China
Abstract：Objective To investigate the effects of microRNA-22 on autophagy in human articular chondrocytes and its role in OA pathogenesis. Methods Primary human articular chondrocytes were obtained from cartilage tissue donated by patients. Real time PCR was used to detect the expression level of microRNA -22 in healthy chondrocytes and osteoarthritis (OA) chondrocytes. Western blot was used to detect the expression of LC3B-Ⅱ、LC3B-Ⅰ in healthy chondrocytes and OA chondrocytes and the ratio of LC3B-Ⅱ/Ⅰ was calculated. After chondrocytes were transfected with miR-22 mimics and miR-22 inhibitor, colony formation assay was performed to evaluate cell viability and Western blot was used to detect the expression of LC3B-Ⅱ, LC3B-Ⅰ and Collagen Ⅱ. Results Real-time PCR suggested that the expression level of microRNA -22 in OA chondrocytes (2.50±0.39) was significantly higher compared with healthy chondrocytes (0.98±0.25). Western blot results showed that LC3B-Ⅱ was inhibited in OA chondrocytes. The ratio of LC3B-Ⅱ/Ⅰ in OA chondrocytes (1.25±0.13) was significantly lower compared with healthy chondrocytes (1.85±0.21). Results from colony formation assay demonstrated that chondrocytes transfected with miR-22 mimics formed less cell colonies (75.67±11.36) than NC group (107.33±14.3) while miR-22 inhibitor transfection promoted chondrocytes formed more cell colonies (128.5±9.95). Western blot results showed that miR-22 mimics transfection significantly reduced the expression level of LC3B-Ⅱ (1.50±0.20), the ratio of LC3B-Ⅱ/Ⅰ (1.45±0.13) and the expression level of Collagen Ⅱ (0.51±0.09) in chondrocyte while miR-22 inhibitor transfection significantly promoted the expression level of LC3B-Ⅱ (2.36±0.19), the ratio of LC3B-Ⅱ/Ⅰ (2.30±0.08) and the expression level of Collagen Ⅱ (1.31±0.03). Conclusions MicroRNA-22 plays a crucial role in OA pathogenesis through inhibiting chondrocytes autophagy and viability and could be a novel therapeutic target of OA.
颜世举, 董文静, 李志锐, 赵燕鹏, 韩涛, 魏均强, 王俊良, 林峰. microRNA-22对调控软骨细胞自噬的影响及其在骨关节炎发病中的作用机制[J]. 武警医学, 2022, 33(11): 982-986.
YAN Shiju, DONG Wenjing, LI Zhirui, ZHAO Yanpeng, HAN Tao, WEI Junqiang, WANG Junliang, LIN Feng. Role of microRNA-22 regulating chondrocyte autophagy in pathogenesis of osteoarthritis. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(11): 982-986.
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