孟德尔随机化方法探究免疫细胞标记物与子宫内膜异位症的关系

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摘要目的通过孟德尔随机化方法探讨免疫细胞标记物与子宫内膜异位症(EMs)之间的因果关系。方法采用双样本孟德尔随机化分析,评估731个免疫细胞表型与EMs间的因果关系,使用逆方差加权法并进行敏感性分析验证结果的可靠性。结果 9个免疫表型与EMs患病风险增加显著相关,包括CD127 on T cell (OR=1.063, 95%CI:1.001~1.130, P=0.047), CD25 on CD28⁺ CD4⁺(OR=1.149, 95%CI:1.026~1.286, P=0.016), CD28 on CD39⁺ activated Treg (OR=1.004, 95%CI:1.015~1.081, P=0.004), CD33br HLA DR⁺ CD14^-%CD33br HLA DR⁺ (OR=1.035, 95%CI:1.002~1.069, P=0.036), CD45 on lymphocyte (OR=1.059, 95%CI:1.011~1.109, P=0.015), CD45 on NK cell(OR=1.042, 95%CI:1.001~1.086, P=0.046), CM CD8br AC(OR=1.060, 95%CI:1.001~1.122, P=0.046),Granulocyte AC(OR=1.060, 95%CI:1.004~1.120, P=0.035)及HLA DR⁺NK%NK cell (OR=1.044, 95%CI:1.000~1.090, P=0.049)。4个免疫表型与EMs患病风险降低显著相关,CD8 on TD CD8br(OR=0.948, 95%CI:0.903~0.996, P=0.034), DN (CD4^-CD8^-) %leukocyte (OR=0.959, 95%CI:0.930~0.989, P=0.008),HLA DR on HLA DR⁺ CD8br (OR=0.933, 95%CI:0.882~0.988, P=0.018),HLA DR⁺T cell AC(OR=0.972, 95%CI:0.946~0.999, P=0.039)及Naive DN (CD4^-CD8^-)%DN (OR=0.935, 95%CI:0.878~0.995, P=0.035)。敏感性分析的结果与主要研究结果一致。结论免疫细胞与EMs易感性之间有因果关系,为临床决策和药物开发提供了新方向。

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关键词 ：子宫内膜异位症, 免疫细胞, 孟德尔随机化探究, 危险作用, 保护作用

Abstract：Objective To investigate the causal relationship between immune cell markers and endometriosis (EMs) by Mendelian randomization method. Methods Two-sample Mendelian randomization analysis (MR) was used to evaluate the causal relationship between 731 immune cell phenotypes and EMs. Inverse variance weighting method was used and sensitivity analysis was performed to verify the reliability of the results. Results Nine immunophenotypes were significantly associated with increased risk of EMs, including CD127 on T cell (OR=1.063, 95%CI:1.001-1.130, P=0.047), CD25 on CD28⁺ CD4⁺(OR=1.149, 95%CI:1.026-1.286, P=0.016), CD28 on CD39+ activated Treg (OR=1.004, 95%CI:1.015-1.081, P=0.004), CD33br HLA DR⁺ CD14%CD33br HLA DR⁺ (OR=1.035, 95%CI:1.002-1.069, P=0.036), CD45 on lymphocyte (OR=1.059, 95%CI:1.011-1.109, P=0.015), CD45 on NK cell(OR=1.042, 95%CI:1.001-1.086, P=0.046), CM CD8br AC(OR=1.060, 95%CI:1.001-1.122, P=0.046),Granulocyte AC(OR=1.060, 95%CI:1.004-1.120, P=0.035) and HLA DR⁺NK%NK cell (OR=1.044, 95%CI:1.000-1.090, P=0.049. Four immunophenotypes were significantly associated with reduced risk of EMs, , including CD8 on TD CD8br (OR=0.948, 95%CI:0.903-0.996, P=0.034), DN (CD4^- CD8^-) %leukocyte (OR=0.959, 95%CI:0.930-0.989, P=0.008),HLA DR on HLA DR⁺ CD8br (OR=0.933, 95%CI:0.882-0.988, P=0.018),HLA DR⁺T cell AC(OR=0.972, 95%CI:0.946-0.999, P=0.039), and Naive DN (CD4^-CD8^-) %DN (OR=0.935, 95%CI:0.878-0.995, P=0.035). The results of sensitivity analysis were consistent with the results of the main study. Conclusions There is causal relationship between immune cells and susceptibility to EMs, which provides a new direction for clinical decision making and drug development.

Key words： endometriosis immune cell Mendelian randomization inquiry risk effect protective effect

收稿日期: 2024-06-24

ZTFLH:

R711.71

基金资助:首都科研发展专项项目(2020-1-5112)

通讯作者: 张晓莉,E-mail: wjzhangxiaoli@sina.cn

作者简介: 李佳璐,硕士,医师。

引用本文:

李佳璐, 杨悦, 张晓莉. 孟德尔随机化方法探究免疫细胞标记物与子宫内膜异位症的关系[J]. 武警医学, 2025, 36(2): 145-149. LI Jialu, YANG Yue, ZHANG Xiaoli. Relationship between immune cell markers and endometriosis explored by Mendelian Randomization method. Med. J. Chin. Peop. Armed Poli. Forc., 2025, 36(2): 145-149.

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https://journal08.magtechjournal.com/Jwk_wjyx/CN/ 或 https://journal08.magtechjournal.com/Jwk_wjyx/CN/Y2025/V36/I2/145

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