Abstract: This paper mainly researched the impact of ambidextrous routines on cooperative innovation performance, and did an empirical analysis in different culture contexts. On the basis of the literature review and theoretical analysis, we constructed the theoretical model, surveyed enterprises with strong R&D cooperation intensity and depth in the province of Shaanxi, Jiangsu and Guangdong, by field research and empirical analysis, the results show that, ambidextrous routines has different influences on the cooperative innovation performance in different culture contexts. In the three samples, there was significant differences between the relationship of exploitation routines and cooperative innovation performance; and there was an inverted U-shaped relationship between exploration routines and cooperative innovation performance in all three samples.