Abstract: The paper documents the case of Carpoly Chemical Enterprise and structures a mechanism model due to the duality influence of university-industry cooperation to firm knowledge structure, which is complementary and supplementary dimensions impact. According to the analysis of Carpoly, this paper find there is a contradictory relationship between breadth and depth of firm technology structure account of the different introductions of technology. Meanwhile， as the development of self-dependent innovation abilities : the dependency to university-industry cooperation of enterprise will reduce， the main part of university-industry will convert from application technology to basic technology， the whole process of technology introduction type of enterprise will meet the change of “supplymentery technology” to “supplymentery technology and complementary technology” to “complementary technology”.