Abstract: Based on provincial panel data of in China from 2005 to 2012, this paper explores the impact of high-tech industrial agglomeration on technological innovation and its regional comparison. The theoretical analysis show that the mechanisms of the impact of high-tech industrial agglomeration on technological innovation are that high-tech industrial agglomeration can help to promote the generation of technological innovation and diffusion of technological innovation. Empirical analysis show that under the condition that scientific & technical personnel and funds, FDI as well as institutional innovation, the high-tech industrial agglomeration can promote technological innovation; Region comparison reveals that impact of high-tech industrial agglomeration on technological innovation of inter-regional are different: the impact of high-tech industrial agglomeration on technological innovation in eastern region is greater than the central & western regions. The policy enlightenments of this paper are that cultivating high-tech industrial cluster, Playing a role in promoting talent and capital on technological innovation, Developing the technological spillover effect of foreign capital utilization, as well as deepening the economic system reform have important significance on promoting high-tech industrial technological innovation.