Abstract: Knowledge interaction of Industrial Technology Innovation Alliances shows the characteristics of industry-oriented ecological evolution. the theory of knowledge management , game theory and ecology are integrated to analyze this characteristics. The ecological relationship of Knowledge interaction in Industrial Technology Innovation Alliances is classified into three kinds such as interaction among Alliance members, interaction between government and Alliance and government- Alliance- members interaction. The relative three kinds of Knowledge interaction relationship models are set up based on revised Lotka-Volterra mode. And the Quantitative analysis and numerical simulation has been made. The results show that the factors like Knowledge absorptive capacity and the largest stock of knowledge have a positive feedback effect on Knowledge growth and balance. High efficiency equilibrium of Knowledge interaction can be achieved if the government injected the power of Knowledge growth into the Alliance and built the ecology of Reciprocal symbiosis and Commensalism.