Abstract: Regional innovation construction is an important task to achieve high-quality development and implement the national innovation driven strategy. Based on symbiont theory framework of innovation system, this paper proposes an integrated analysis framework to describe the differences of regional innovation development. FSQCA is used to analyze the configuration of 31 provinces in China. The results reveal that internet development (symbiotic matrix) and industrial agglomeration (symbiotic network) are the necessary conditions for the high level development of regional innovation, and the lack of symbiotic environment is the necessary conditions for the non-high level development of regional innovation. There are three driving paths of high level development of regional innovation and two driving paths of non-high level development of regional innovation between which have asymmetric relationship. In the high-level development path of regional innovation, the full play of symbiotic network function depends on specific symbiotic environment. Under certain conditions, the symbiotic matrix, symbiotic network and symbiotic environment can achieve "the same result through different ways" by equivalent substitution.