Abstract: At present,the security and stability of the global manufacturing industry chain are facing unprecedented challenges.How to improve the resilience of the industry chain and strengthen the construction of the industry chain has become an urgent problem to be solved.Based on the balanced panel data of 30 provinces and cities in China from 2007 to 2017,from the perspective of industrial transformation and upgrading and innovation chain construction,this paper studies the impact of collaborative agglomeration of manufacturing industry and science and technology service industry (hereinafter referred to as collaborative agglomeration of two industries) on the resilience of manufacturing industry chain,and discusses how the collaborative agglomeration of two industries affects the resilience of manufacturing industry chain by influencing technological innovation,On this basis,we further explore the regulatory role of innovation ecosystem symbiosis in this mechanism.The research finds that: the collaborative agglomeration of two industries can significantly promote the resilience of the manufacturing industry chain by influencing technological innovation.The symbiosis of innovation ecosystem can effectively adjust the impact of the collaborative agglomeration of two industries on the resilience of the manufacturing industry chain.The collaborative agglomeration of two industries can not only directly affect the toughness of the manufacturing industry chain,but also effectively adjust the impact of technological innovation on the toughness of the manufacturing industry chain.Based on the above research conclusions,relevant suggestions are put forward to enhance the resilience of the manufacturing industry chain.